Temporal/spatial expression of nuclear receptor coactivators in the mouse lung

Angela Naltner, Susan Wert, Jeffrey A. Whitsett, Cong Yan

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Our laboratory has previously demonstrated that retinoic acid nuclear receptor, thyroid transcription factor-1 (TTF-1), and nuclear receptor coactivators such as cAMP response element binding protein (CREB) binding protein (CBP)/p300 and steroid receptor coactivator-1 (SRC-1) form an enhanceosome on the 5′-enhancer region of the human surfactant protein B gene. Immunohistochemistry was used to identify cells that coexpressed CBP/p300, SRC-1, retinoid X receptor, and TTF-1 in the developing and mature lung. CBP/p300 and SRC-1 were expressed in the adult mouse lung, CBP and p300 being present in both alveolar type I and type II epithelial cells and SRC-1 and TTF-1 being restricted to type II epithelial cells. CBP/p300, SRC-1, and TTF-1 were readily detected in the nuclei of developing respiratory epithelial tubules in fetal mice from embryonic days 10 to 18. CBP/p300 and SRC-1 were also detected in developing mesenchymal cells. These coactivators were coexpressed with TTF-1 and SP-B in human pulmonary adenocarcinoma cells (H441 cells) in vitro. Interaction assays with a two-hybrid reporter analysis demonstrated direct interactions among TTF-1, SRC-1, and CBP/p300 in H441 cells. These findings support a role for retinoic acid receptor and nuclear receptor coactivators in the regulation of SP-B gene expression in the respiratory epithelium.

Original languageEnglish (US)
Pages (from-to)L1066-L1074
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume279
Issue number6 23-6
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

Keywords

  • Cyclic adenosine 5′-monophosphate response element binding protein binding protein/p300
  • Lung development
  • Steroid receptor coactivator-1
  • Surfactant protein B
  • Thyroid transcription factor-1

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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