Temporospatial expression and cellular localization of oligodendrocyte myelin glycoprotein (OMgp) after traumatic spinal cord injury in adult rats

Fangfang Dou, Lidong Huang, Panpan Yu, Huiqing Zhu, Xiaofei Wang, Jian Zou, Peihua Lu, Xiao-Ming Xu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Traumatic spinal cord injury (SCI) leads to permanent neurological deficits, which, in part, is due to the inability of mature axons to regenerate in the mammalian central nervous system (CNS). The oligodendrocyte myelin glycoprotein (OMgp) is one of the myelin-associated inhibitors of neurite outgrowth in the CNS. To date, limited information is available concerning its expression following SCI, possibly due to the lack of a reliable antibody against it. Here we report the generation of a highly specific OMgp polyclonal antibody from the rabbit. Using this antibody, we found that OMgp was almost exclusively expressed in the CNS. Following a moderately contusive SCI using a New York University impactor (10g rod dropped from a height of 12.5mm), both OMgp mRNA and protein levels were elevated at 1 and 7 days post-SCI, respectively, and peaked at 28 days compared to those of the sham-operated controls. Spatially, OMgp was expressed throughout the entire rostrocaudal extension of a 10mm long spinal segment with the highest expression seen at the injury epicenter. OMgp was exclusively localized in neurons and oligodendrocytes in the normal and sham-operated controls with an increased expression found in these cells following SCI. OMgp was not expressed in astrocytes or microglia in all groups. Thus, our study has provided evidence for temporospatial expression and cellular localization of OMgp following SCI and suggested that this molecule may contribute to the overall inhibition of axonal regeneration.

Original languageEnglish
Pages (from-to)2299-2311
Number of pages13
JournalJournal of Neurotrauma
Volume26
Issue number12
DOIs
StatePublished - Dec 1 2009

Fingerprint

Oligodendrocyte-Myelin Glycoprotein
Spinal Cord Injuries
Central Nervous System
Antibodies
Oligodendroglia
Microglia
Myelin Sheath
Astrocytes
Axons
Regeneration

Keywords

  • Myelin-associated inhibitor
  • Neuron
  • Oligodendrocyte
  • OMgp
  • Polyclonal antibody
  • Spinal cord injury

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Temporospatial expression and cellular localization of oligodendrocyte myelin glycoprotein (OMgp) after traumatic spinal cord injury in adult rats. / Dou, Fangfang; Huang, Lidong; Yu, Panpan; Zhu, Huiqing; Wang, Xiaofei; Zou, Jian; Lu, Peihua; Xu, Xiao-Ming.

In: Journal of Neurotrauma, Vol. 26, No. 12, 01.12.2009, p. 2299-2311.

Research output: Contribution to journalArticle

Dou, Fangfang ; Huang, Lidong ; Yu, Panpan ; Zhu, Huiqing ; Wang, Xiaofei ; Zou, Jian ; Lu, Peihua ; Xu, Xiao-Ming. / Temporospatial expression and cellular localization of oligodendrocyte myelin glycoprotein (OMgp) after traumatic spinal cord injury in adult rats. In: Journal of Neurotrauma. 2009 ; Vol. 26, No. 12. pp. 2299-2311.
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