Tensional forces influence gene expression and sutural state of rat calvariae in vitro

Sunil S. Tholpady, Toby F. Freyman, Debbie Chachra, Roy C. Ogle

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

BACKGROUND: Theories regarding the cause of craniosynostosis that are more than 15 years old cite the role that tensional forces play in the normal and abnormal development of the cranial suture. These theories highlight the effect of stress bands originating from the skull base to the vertex, guiding sutural development. METHODS: In this study, the normally fusing posterior intrafrontal suture of the rat was subjected to 3 mN of tensional force for 30 minutes per day. The suture was then assessed for patency, proliferation, apoptosis, and transforming growth factor (TGF)-β signaling components. RESULTS: Sutures that were subjected to tensional force were histologically patent at the end of 14 days. This was in contrast to sutures that were maintained without force. Proliferative and apoptotic activity was increased also in sutures maintained open artificially. Interestingly, levels of active TGF-β-signaling components were also increased in force-maintained sutures. CONCLUSIONS: Sutural maintenance by mechanical force is concurrent with modulation of cellular activity and protein expression reminiscent of the open suture. This study demonstrates the dynamic reciprocity existing between biochemical activity and morphologic state. Although it is known that changes in TGF-βs and fibroblast growth factors can cause sutural fusion, this is the first study to demonstrate that abrogation of sutural closure is responsible for growth factor signaling modulation.

Original languageEnglish (US)
Pages (from-to)601-611
Number of pages11
JournalPlastic and Reconstructive Surgery
Volume120
Issue number3
DOIs
StatePublished - Sep 2007
Externally publishedYes

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Skull
Sutures
Gene Expression
Transforming Growth Factors
Cranial Sutures
Craniosynostoses
Fibroblast Growth Factors
Skull Base
In Vitro Techniques
Intercellular Signaling Peptides and Proteins
Maintenance
Apoptosis
Proteins

ASJC Scopus subject areas

  • Surgery

Cite this

Tensional forces influence gene expression and sutural state of rat calvariae in vitro. / Tholpady, Sunil S.; Freyman, Toby F.; Chachra, Debbie; Ogle, Roy C.

In: Plastic and Reconstructive Surgery, Vol. 120, No. 3, 09.2007, p. 601-611.

Research output: Contribution to journalArticle

Tholpady, Sunil S. ; Freyman, Toby F. ; Chachra, Debbie ; Ogle, Roy C. / Tensional forces influence gene expression and sutural state of rat calvariae in vitro. In: Plastic and Reconstructive Surgery. 2007 ; Vol. 120, No. 3. pp. 601-611.
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