Terahertz pulsed imaging study to assess remineralization of artificial caries lesions

David Churchley, Richard J M Lynch, Frank Lippert, Jennifer Susan O Bryan Eder, Jesse Alton, Carlos Gonzalez-Cabezas

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We compare terahertz-pulsed imaging (TPI) with transverse microradiography (TMR) and microinden-tation to measure remineralization of artificial caries lesions. Lesions are formed in bovine enamel using a solution of 0.1 M lactic acid/0.2% Carbopol C907 and 50% saturated with hydroxyapatite adjusted to pH 5.0. The 20-day experimental protocol consists of four 1 min treatment periods with dentifrices containing 10, 675, 1385, and 2700 ppm fluoride, a 4-h/day acid challenge, and, for the remaining time, specimens are stored in a 50:50 pooled human/artificial saliva mixture. Each specimen is imaged at the focal point of the terahertz beam (data-point spacing = 50 μm). The time-domain data are used to calculate the refractive index volume percent profile throughout the lesion, and the differences in the integrated areas between the baseline and post-treatment profiles are used to calculate ΔΔZ (THz). In addition, the change from baseline in both the lesion depth and the intensity of the reflected pulse from the air/enamel interface is determined. Statistically significant Pearson correlation coefficients are observed between TPI and TMR/microindentation (P <0.05). We demonstrate that TPI has potential as a research tool for hard tissue imaging.

Original languageEnglish (US)
Article number026001
JournalJournal of Biomedical Optics
Volume16
Issue number2
DOIs
StatePublished - Feb 2011

Fingerprint

lesions
Imaging techniques
enamels
Enamels
Artificial Saliva
Dentifrices
saliva
lactic acid
Durapatite
Lactic acid
profiles
Fluorides
Hydroxyapatite
correlation coefficients
microhardness
fluorides
Lactic Acid
Refractive index
spacing
Tissue

Keywords

  • Caries
  • Microindentation
  • Microradiography
  • Remineralization
  • Terahertz imaging

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Churchley, D., Lynch, R. J. M., Lippert, F., Eder, J. S. O. B., Alton, J., & Gonzalez-Cabezas, C. (2011). Terahertz pulsed imaging study to assess remineralization of artificial caries lesions. Journal of Biomedical Optics, 16(2), [026001]. https://doi.org/10.1117/1.3540277

Terahertz pulsed imaging study to assess remineralization of artificial caries lesions. / Churchley, David; Lynch, Richard J M; Lippert, Frank; Eder, Jennifer Susan O Bryan; Alton, Jesse; Gonzalez-Cabezas, Carlos.

In: Journal of Biomedical Optics, Vol. 16, No. 2, 026001, 02.2011.

Research output: Contribution to journalArticle

Churchley, David ; Lynch, Richard J M ; Lippert, Frank ; Eder, Jennifer Susan O Bryan ; Alton, Jesse ; Gonzalez-Cabezas, Carlos. / Terahertz pulsed imaging study to assess remineralization of artificial caries lesions. In: Journal of Biomedical Optics. 2011 ; Vol. 16, No. 2.
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