Testing effects of signal transduction pathways on cadherin junctional complex assembly using quantitative image analysis

Shobha Gopalakrishnan, Kenneth W. Dunn, James A. Marrs

Research output: Contribution to journalArticle

Abstract

Cadherin adhesion molecules function in numerous cell biological processes that influence embryo development, normal cell physiology, and pathophysiology of many disease processes. Cadherins nucleate the assembly of the adherens junction, a cell-to-cell adhesion plaque that is prominent in simple epithelial cells and found in many cell types. Numerous cell biological approaches have been used to study this interesting class of molecules. Here, we outline methodology used in our studies of junctional complexes to examine effects of signaling molecules on assembly mechanisms. This is a quantitative method that allows the investigator to test the combined effect of two different signaling processes to determine whether these two signals act in concert within the same pathway. We discuss how this method could be generalized to other studies to examine consequences of various experimental manipulations on the assembly of cellular structures.

Original languageEnglish (US)
Pages (from-to)218-227
Number of pages10
JournalMethods
Volume30
Issue number3
DOIs
StatePublished - Jul 1 2003

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ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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