Testosterone Delivered with a Scaffold Is as Effective as Bone Morphologic Protein-2 in Promoting the Repair of Critical-Size Segmental Defect of Femoral Bone in Mice

Bi Hua Cheng, T.M. Gabriel Chu, Chawnshang Chang, Hong Yo Kang, Ko En Huang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Loss of large bone segments due to fracture resulting from trauma or tumor removal is a common clinical problem. The goal of this study was to evaluate the use of scaffolds containing testosterone, bone morphogenetic protein-2 (BMP-2), or a combination of both for treatment of critical-size segmental bone defects in mice. A 2.5-mm wide osteotomy was created on the left femur of wildtype and androgen receptor knockout (ARKO) mice. Testosterone, BMP-2, or both were delivered locally using a scaffold that bridged the fracture. Results of X-ray imaging showed that in both wildtype and ARKO mice, BMP-2 treatment induced callus formation within 14 days after initiation of the treatment. Testosterone treatment also induced callus formation within 14 days in wildtype but not in ARKO mice. Micro-computed tomography and histological examinations revealed that testosterone treatment caused similar degrees of callus formation as BMP-2 treatment in wildtype mice, but had no such effect in ARKO mice, suggesting that the androgen receptor is required for testosterone to initiate fracture healing. These results demonstrate that testosterone is as effective as BMP-2 in promoting the healing of critical-size segmental defects and that combination therapy with testosterone and BMP-2 is superior to single therapy. Results of this study may provide a foundation to develop a cost effective and efficient therapeutic modality for treatment of bone fractures with segmental defects.

Original languageEnglish
Article numbere70234
JournalPLoS One
Volume8
Issue number8
DOIs
StatePublished - Aug 5 2013

Fingerprint

bone morphogenetic proteins
thighs
Thigh
Scaffolds
testosterone
Bone Morphogenetic Protein 2
Testosterone
Bone
Repair
Androgen Receptors
bones
Bone and Bones
Defects
mice
bone formation
Proteins
proteins
Knockout Mice
Bony Callus
therapeutics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Testosterone Delivered with a Scaffold Is as Effective as Bone Morphologic Protein-2 in Promoting the Repair of Critical-Size Segmental Defect of Femoral Bone in Mice. / Cheng, Bi Hua; Chu, T.M. Gabriel; Chang, Chawnshang; Kang, Hong Yo; Huang, Ko En.

In: PLoS One, Vol. 8, No. 8, e70234, 05.08.2013.

Research output: Contribution to journalArticle

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