Testosterone enhancement of the nerve cell body response to injury: Evidence using in situ hybridization and ribosomal DNA probes

Nancy B. Kinderman, Kathryn Jones

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

In axotomized peripheral motoneurons capable of successful regeneration, one of the earliest morphological indicators of the injury response occurs within the nucleolus. In the initial part of this investigation, we mapped the nucleolar response of injured adult hamster facial motoneurons from a molecular perspective, utilizing in situ hybridization and ribosomal DNA probes complementary to stable rRNA. Recently, we have discovered that the gonadal steroid, testosterone propionate (TP), accelerates recovery from facial paralysis in the hamster by increasing the rate of regeneration of the fastest regrowing axons. In the second part of this study, the hypothesis that TP accomplishes these effects on facial nerve regeneration through an enhancement of the nerve cell body response to injury was tested using in situ hybridization and rDNA probes. Adult intact male hamliters were subjected to right facial nerve axotomies at the stylomastoid foramen. One-half of the axotomized animals received subcutaneous implants of TP, with the remainder sham implanted. In situ hybridization with tritiated rDNA probes was accomplished and levels of hybridizable rRNA assessed both qualitatively and quantitatively. Axotomy alone induced an upregulation in rRNA levels, with peak changes occurring by 24 hr postoperative and continuing through Postoperative day 4. These molecular changes in the nucleofar response preceded, by a full day, any morphological signs of the nucleolar reactive pattern previously found in this cell type, and, as such, point to the usefulness of in situ hybridization as a tool to identify the earliest events associated with the axon reaction. A secondary smaller increase in rRNA levels was observed during the later stages of reparation. TP significantly augmented the ribosomal response to injury, with levels of rRNA increased as early as 6 hr and the magnitude of the response greater than that occurring following axotomy alone. These results provide the first mechanistic step in the identification of the cellular processes underlying gonadal steroid augmentation of neuronal reparative processes. We conclude that TP accelerates the "switch" from a normal to a reparative state and suggest that this that this priming effect may be causally related to the differential effects of TP on the regenerative properties of this cell type.

Original languageEnglish (US)
Pages (from-to)1523-1532
Number of pages10
JournalJournal of Neuroscience
Volume13
Issue number4
StatePublished - 1993
Externally publishedYes

Fingerprint

Testosterone Propionate
DNA Probes
Ribosomal DNA
In Situ Hybridization
Testosterone
Neurons
Axotomy
Wounds and Injuries
Facial Nerve
Motor Neurons
Cricetinae
Regeneration
Steroids
Retrograde Degeneration
Nerve Regeneration
Facial Paralysis
Axons
Cell Body
Up-Regulation

Keywords

  • Gonadal steroid
  • Nerve regeneration
  • Neurotrophic agent
  • Nucleolar reactive pattern

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Testosterone enhancement of the nerve cell body response to injury : Evidence using in situ hybridization and ribosomal DNA probes. / Kinderman, Nancy B.; Jones, Kathryn.

In: Journal of Neuroscience, Vol. 13, No. 4, 1993, p. 1523-1532.

Research output: Contribution to journalArticle

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