TGF-β increases retinal endothelial cell permeability by increasing MMP-9

Possible role of glial cells in endothelial barrier function

M. A. Behzadian, X. L. Wang, L. Windsor, N. Ghaly, R. B. Caldwell

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Purpose. To determine transforming growth factor (TGF) β effects on matrix metalloproteinases (MMPs) as a potential cause of the blood-retinal barrier breakdown at the onset of angiogenesis. Previously, glial cells were shown to play a role in the angiogenesis process and to express the angiogenic regulating factor TGF-β, which becomes active under hypoxia conditions. Here, the authors demonstrate that retinal endothelial cells express MMP-9 when treated with TGF-β or cocultured with glial cells and that both TGF-β and MMP-9 increase endothelial cell permeability. Methods. Primary cultures of bovine retinal endothelial (BRE) cells grown on porous membranes were treated with TGF-β or purified MMP-9, and permeability changes were assayed. The amount and distribution of the tight junction protein occludin also was analyzed by immunocytochemistry and Western blotting. Cell extracts or conditioned media from TGF-β-treated BRE cells and from glial cell-BRE cocultures were analyzed for MMP-9 content by substrate gel electrophoresis (zymography) or Western blotting. Results. Both TGF-β and MMP-9 increased the permeability of BRE monolayers and reduced the levels of the junction protein occludin. The effect of MMP-9 on permeability was rapid, but the TGF-β-induced permeability required longer incubation and was blocked by anti-TGF-β and anti-MMP-9 antibodies as well as by TGF-β latency-associated peptide. Zymography showed that MMP-9 activity, which was very low or absent in untreated BRE cultures, was dramatically increased by TGF-β as well as by coculturing with either astrocytes or Müller glial cells, Anti-TGF-β antibody blocked the TGF-β effect, but not the coculture effect on MMP-9 production. Conclusions. These data indicate a direct correlation between TGF-β-induced MMP-9 activity and increased endothelial cell permeability, Moreover, endothelial cell production of MMP-9 is regulated by glial cells through expression of TGF-β or by direct cell-to-cell contact. During retinal disease, glial cell production of active TGF-β may contribute to breakdown of the blood-retinal barrier by stimulating endothelial cell MMP-9 production.

Original languageEnglish (US)
Pages (from-to)853-859
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume42
Issue number3
StatePublished - 2001
Externally publishedYes

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Matrix Metalloproteinase 9
Transforming Growth Factors
Neuroglia
Permeability
Endothelial Cells
Blood-Retinal Barrier
Occludin
Coculture Techniques
Western Blotting
Tight Junction Proteins
Retinal Diseases
Antibodies
Angiogenesis Inducing Agents
Conditioned Culture Medium
Cell Extracts
Matrix Metalloproteinases
Astrocytes

ASJC Scopus subject areas

  • Ophthalmology

Cite this

TGF-β increases retinal endothelial cell permeability by increasing MMP-9 : Possible role of glial cells in endothelial barrier function. / Behzadian, M. A.; Wang, X. L.; Windsor, L.; Ghaly, N.; Caldwell, R. B.

In: Investigative Ophthalmology and Visual Science, Vol. 42, No. 3, 2001, p. 853-859.

Research output: Contribution to journalArticle

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abstract = "Purpose. To determine transforming growth factor (TGF) β effects on matrix metalloproteinases (MMPs) as a potential cause of the blood-retinal barrier breakdown at the onset of angiogenesis. Previously, glial cells were shown to play a role in the angiogenesis process and to express the angiogenic regulating factor TGF-β, which becomes active under hypoxia conditions. Here, the authors demonstrate that retinal endothelial cells express MMP-9 when treated with TGF-β or cocultured with glial cells and that both TGF-β and MMP-9 increase endothelial cell permeability. Methods. Primary cultures of bovine retinal endothelial (BRE) cells grown on porous membranes were treated with TGF-β or purified MMP-9, and permeability changes were assayed. The amount and distribution of the tight junction protein occludin also was analyzed by immunocytochemistry and Western blotting. Cell extracts or conditioned media from TGF-β-treated BRE cells and from glial cell-BRE cocultures were analyzed for MMP-9 content by substrate gel electrophoresis (zymography) or Western blotting. Results. Both TGF-β and MMP-9 increased the permeability of BRE monolayers and reduced the levels of the junction protein occludin. The effect of MMP-9 on permeability was rapid, but the TGF-β-induced permeability required longer incubation and was blocked by anti-TGF-β and anti-MMP-9 antibodies as well as by TGF-β latency-associated peptide. Zymography showed that MMP-9 activity, which was very low or absent in untreated BRE cultures, was dramatically increased by TGF-β as well as by coculturing with either astrocytes or M{\"u}ller glial cells, Anti-TGF-β antibody blocked the TGF-β effect, but not the coculture effect on MMP-9 production. Conclusions. These data indicate a direct correlation between TGF-β-induced MMP-9 activity and increased endothelial cell permeability, Moreover, endothelial cell production of MMP-9 is regulated by glial cells through expression of TGF-β or by direct cell-to-cell contact. During retinal disease, glial cell production of active TGF-β may contribute to breakdown of the blood-retinal barrier by stimulating endothelial cell MMP-9 production.",
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T2 - Possible role of glial cells in endothelial barrier function

AU - Behzadian, M. A.

AU - Wang, X. L.

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AU - Ghaly, N.

AU - Caldwell, R. B.

PY - 2001

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N2 - Purpose. To determine transforming growth factor (TGF) β effects on matrix metalloproteinases (MMPs) as a potential cause of the blood-retinal barrier breakdown at the onset of angiogenesis. Previously, glial cells were shown to play a role in the angiogenesis process and to express the angiogenic regulating factor TGF-β, which becomes active under hypoxia conditions. Here, the authors demonstrate that retinal endothelial cells express MMP-9 when treated with TGF-β or cocultured with glial cells and that both TGF-β and MMP-9 increase endothelial cell permeability. Methods. Primary cultures of bovine retinal endothelial (BRE) cells grown on porous membranes were treated with TGF-β or purified MMP-9, and permeability changes were assayed. The amount and distribution of the tight junction protein occludin also was analyzed by immunocytochemistry and Western blotting. Cell extracts or conditioned media from TGF-β-treated BRE cells and from glial cell-BRE cocultures were analyzed for MMP-9 content by substrate gel electrophoresis (zymography) or Western blotting. Results. Both TGF-β and MMP-9 increased the permeability of BRE monolayers and reduced the levels of the junction protein occludin. The effect of MMP-9 on permeability was rapid, but the TGF-β-induced permeability required longer incubation and was blocked by anti-TGF-β and anti-MMP-9 antibodies as well as by TGF-β latency-associated peptide. Zymography showed that MMP-9 activity, which was very low or absent in untreated BRE cultures, was dramatically increased by TGF-β as well as by coculturing with either astrocytes or Müller glial cells, Anti-TGF-β antibody blocked the TGF-β effect, but not the coculture effect on MMP-9 production. Conclusions. These data indicate a direct correlation between TGF-β-induced MMP-9 activity and increased endothelial cell permeability, Moreover, endothelial cell production of MMP-9 is regulated by glial cells through expression of TGF-β or by direct cell-to-cell contact. During retinal disease, glial cell production of active TGF-β may contribute to breakdown of the blood-retinal barrier by stimulating endothelial cell MMP-9 production.

AB - Purpose. To determine transforming growth factor (TGF) β effects on matrix metalloproteinases (MMPs) as a potential cause of the blood-retinal barrier breakdown at the onset of angiogenesis. Previously, glial cells were shown to play a role in the angiogenesis process and to express the angiogenic regulating factor TGF-β, which becomes active under hypoxia conditions. Here, the authors demonstrate that retinal endothelial cells express MMP-9 when treated with TGF-β or cocultured with glial cells and that both TGF-β and MMP-9 increase endothelial cell permeability. Methods. Primary cultures of bovine retinal endothelial (BRE) cells grown on porous membranes were treated with TGF-β or purified MMP-9, and permeability changes were assayed. The amount and distribution of the tight junction protein occludin also was analyzed by immunocytochemistry and Western blotting. Cell extracts or conditioned media from TGF-β-treated BRE cells and from glial cell-BRE cocultures were analyzed for MMP-9 content by substrate gel electrophoresis (zymography) or Western blotting. Results. Both TGF-β and MMP-9 increased the permeability of BRE monolayers and reduced the levels of the junction protein occludin. The effect of MMP-9 on permeability was rapid, but the TGF-β-induced permeability required longer incubation and was blocked by anti-TGF-β and anti-MMP-9 antibodies as well as by TGF-β latency-associated peptide. Zymography showed that MMP-9 activity, which was very low or absent in untreated BRE cultures, was dramatically increased by TGF-β as well as by coculturing with either astrocytes or Müller glial cells, Anti-TGF-β antibody blocked the TGF-β effect, but not the coculture effect on MMP-9 production. Conclusions. These data indicate a direct correlation between TGF-β-induced MMP-9 activity and increased endothelial cell permeability, Moreover, endothelial cell production of MMP-9 is regulated by glial cells through expression of TGF-β or by direct cell-to-cell contact. During retinal disease, glial cell production of active TGF-β may contribute to breakdown of the blood-retinal barrier by stimulating endothelial cell MMP-9 production.

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