Th9 cell development requires a BATF-regulated transcriptional network

Rukhsana Jabeen, Ritobrata Goswami, Olufolakemi Awe, Aishwarya Kulkarni, Evelyn T. Nguyen, Andrea Attenasio, Daniel Walsh, Matthew R. Olson, Myung H. Kim, Robert Tepper, Jie Sun, Chang H. Kim, Elizabeth J. Taparowsky, Baohua Zhou, Mark Kaplan

Research output: Contribution to journalArticle

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Abstract

T helper 9 (Th9) cells are specialized for the production of IL-9, promote allergic inflammation in mice, and are associated with allergic disease in humans. It has not been determined whether Th9 cells express a characteristic transcriptional signature. In this study, we performed microarray analysis to identify genes enriched in Th9 cells compared with other Th subsets. This analysis defined a transcriptional regulatory network required for the expression of a subset of Th9-enriched genes. The activator protein 1 (AP1) family transcription factor BATF (B cell, activating transcription factor-like) was among the genes enriched in Th9 cells and was required for the expression of IL-9 and other Th9-associated genes in both human and mouse T cells. The expression of BATF was increased in Th9 cultures derived from atopic infants compared with Th9 cultures from control infants. T cells deficient in BATF expression had a diminished capacity to promote allergic inflammation compared with wild-type controls. Moreover, mouse Th9 cells ectopically expressing BATF were more efficient at promoting allergic inflammation than control transduced cells. These data indicate that BATF is a central regulator of the Th9 phenotype and contributes to the development of allergic inflammation.

Original languageEnglish
Pages (from-to)4641-4653
Number of pages13
JournalJournal of Clinical Investigation
Volume123
Issue number11
DOIs
StatePublished - Nov 1 2013

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Activating Transcription Factors
B-Cell Activating Factor
Gene Regulatory Networks
Helper-Inducer T-Lymphocytes
Interleukin-9
Inflammation
Genes
T-Lymphocytes
Transcription Factor AP-1
Microarray Analysis
Transcription Factors
Phenotype

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Jabeen, R., Goswami, R., Awe, O., Kulkarni, A., Nguyen, E. T., Attenasio, A., ... Kaplan, M. (2013). Th9 cell development requires a BATF-regulated transcriptional network. Journal of Clinical Investigation, 123(11), 4641-4653. https://doi.org/10.1172/JCI69489

Th9 cell development requires a BATF-regulated transcriptional network. / Jabeen, Rukhsana; Goswami, Ritobrata; Awe, Olufolakemi; Kulkarni, Aishwarya; Nguyen, Evelyn T.; Attenasio, Andrea; Walsh, Daniel; Olson, Matthew R.; Kim, Myung H.; Tepper, Robert; Sun, Jie; Kim, Chang H.; Taparowsky, Elizabeth J.; Zhou, Baohua; Kaplan, Mark.

In: Journal of Clinical Investigation, Vol. 123, No. 11, 01.11.2013, p. 4641-4653.

Research output: Contribution to journalArticle

Jabeen, R, Goswami, R, Awe, O, Kulkarni, A, Nguyen, ET, Attenasio, A, Walsh, D, Olson, MR, Kim, MH, Tepper, R, Sun, J, Kim, CH, Taparowsky, EJ, Zhou, B & Kaplan, M 2013, 'Th9 cell development requires a BATF-regulated transcriptional network', Journal of Clinical Investigation, vol. 123, no. 11, pp. 4641-4653. https://doi.org/10.1172/JCI69489
Jabeen R, Goswami R, Awe O, Kulkarni A, Nguyen ET, Attenasio A et al. Th9 cell development requires a BATF-regulated transcriptional network. Journal of Clinical Investigation. 2013 Nov 1;123(11):4641-4653. https://doi.org/10.1172/JCI69489
Jabeen, Rukhsana ; Goswami, Ritobrata ; Awe, Olufolakemi ; Kulkarni, Aishwarya ; Nguyen, Evelyn T. ; Attenasio, Andrea ; Walsh, Daniel ; Olson, Matthew R. ; Kim, Myung H. ; Tepper, Robert ; Sun, Jie ; Kim, Chang H. ; Taparowsky, Elizabeth J. ; Zhou, Baohua ; Kaplan, Mark. / Th9 cell development requires a BATF-regulated transcriptional network. In: Journal of Clinical Investigation. 2013 ; Vol. 123, No. 11. pp. 4641-4653.
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