The 3′-5′ exonuclease of human DMA polymerase δ (pol δ) is regulated by pol δ accessory factors and deoxyribonucleoside triphosphates

Suk-Hee Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Human DMA polymerase δ (pol δ) is required for the synthesis of leading strand of simian virus 40 (SV40) DNA replication in vitro. Pol δ requires the accessory factors, proliferating cell nuclear antigen (PCNA), activator 1 (A1 ; also known as replication factor C [RFC]), human single-stranded DNA binding protein (HSSB; also known as replication protein A [RP-A]) for the elongation of primed template DNA. Since pol δ has an associated 3′ - 5′ exonuclease activity, the effect of pol δ accessory factors on the exonuclease activity was examined. The 3′-5′ exonuclease activity was stimulated 8-10 fold by the addition of HSSB, and this stimulatory effect was preferential to HSSB since other SSBs from E.coli, T4 or adenovirus, had a little or no effect. The stimulatory effect of HSSB was markedly inhibited by the combined action of A1 and PCNA. Furthermore, the addition of deoxyribonucleoside triphosphates (dNTPs) completely abolished the effect of HSSB on the 3′ - 5′ exonuclease activity even in the absence of pol δ accessory factors. These results suggest that accessory factors and dNTPs regulate both the polymerase and the 3′-5 exonuclease activities.

Original languageEnglish (US)
Pages (from-to)1935-1939
Number of pages5
JournalNucleic Acids Research
Volume21
Issue number8
StatePublished - 1993
Externally publishedYes

Fingerprint

Deoxyribonucleosides
Exonucleases
Accessories
Dynamic mechanical analysis
DNA
Antigens
Proliferating Cell Nuclear Antigen
Replication
Replication Protein C
Replication Protein A
DNA-binding Protein
DNA Replication
Viruses
Simian virus 40
Escherichia coli
Cell
DNA-Binding Proteins
Elongation
Adenoviridae
Escherichia Coli

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

The 3′-5′ exonuclease of human DMA polymerase δ (pol δ) is regulated by pol δ accessory factors and deoxyribonucleoside triphosphates. / Lee, Suk-Hee.

In: Nucleic Acids Research, Vol. 21, No. 8, 1993, p. 1935-1939.

Research output: Contribution to journalArticle

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