The activity of a blood type B specific exoglycosidase from Glycine max

Lois Hobbs, Moonmoon Mitra, Roy Phillips, Dan Smith

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Soluble antigens, enzyme-linked immunosorbent assays (ELISA), and cell suspension assays were used to study the blood group B activity of Glycine max (soybean) α-D-galactosidase. The enzyme readily hydrolyzed the terminal α-D-galactosyl of the B antigen under a variety of conditions, converting it to H antigen. Conversion of the B antigen to H antigen produces blood type O which is universally transfusable. These preliminary studies are important in determining optimal conditions for enzymatic conversion of blood type B to O erythrocytes if efficient large-scale production of enzymatically converted, universally transfusable red blood cells is to be achieved.

Original languageEnglish (US)
Pages (from-to)7-21
Number of pages15
JournalClinica Chimica Acta
Volume247
Issue number1-2
DOIs
StatePublished - Mar 29 1996
Externally publishedYes

Keywords

  • Coffea canephora
  • ELISA
  • Glycine max
  • blood type

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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