The advantages of an attenuated total internal reflection infrared microspectroscopic imaging approach for kidney biopsy analysis

Heather J. Gulley-Stahl, Sharon B. Bledsoe, Andrew Evan, André J. Sommer

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The benefits of an attenuated total reflection Fourier transform infrared (ATR-FTIR) imaging approach for kidney biopsy analysis are described. Biopsy sections collected from kidney-stone formers are analyzed at the initial stages of stone development to provide insights into stone growth and formation. The majority of tissue analysis currently conducted with IR microspectroscopy is performed with a transflection method. The research presented in this manuscript demonstrates that ATR overcomes many of the disadvantages of transflection or transmission measurements for tissue analysis including an elimination of spectral artifacts. When kidney biopsies with small mineral inclusions are analyzed with a transflection approach, specular reflection and the Christiansen effect (anomalous dispersion) can occur, leading to spectral artifacts. Another effect specific to the analysis of mineral inclusions present in kidney biopsies is known as the reststrahlen effect whereby the inclusions become strong reflectors near an absorption band. ATR eliminates these effects by immersing the sample in a high index medium. Additionally, the focused beam size for ATR is decreased by a factor of four when a germanium internal reflection element is used, allowing the acquisition of spectra from small mineral inclusions several micrometers in diameter. If quantitative analysis of small mineral inclusions is ultimately desired, ATR provides the photometrically accurate spectra necessary for quantification.

Original languageEnglish
Pages (from-to)15-22
Number of pages8
JournalApplied Spectroscopy
Volume64
Issue number1
StatePublished - Jan 2010

Fingerprint

Biopsy
infrared reflection
Infrared imaging
kidneys
Minerals
inclusions
Kidney
minerals
Artifacts
artifacts
Germanium
Kidney Calculi
kidney stones
rocks
Tissue
Fourier Analysis
specular reflection
quantitative analysis
reflectors
micrometers

Keywords

  • ATR-FTIR imaging
  • Attenuated total reflection
  • Fourier transform infrared imaging
  • Kidney biopsy
  • Kidney stone disease
  • Tissue analysis
  • Transflection spectroscopy

ASJC Scopus subject areas

  • Spectroscopy
  • Instrumentation
  • Medicine(all)

Cite this

The advantages of an attenuated total internal reflection infrared microspectroscopic imaging approach for kidney biopsy analysis. / Gulley-Stahl, Heather J.; Bledsoe, Sharon B.; Evan, Andrew; Sommer, André J.

In: Applied Spectroscopy, Vol. 64, No. 1, 01.2010, p. 15-22.

Research output: Contribution to journalArticle

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