The application of differential display as a gene profiling tool.

Ken Chien Neng Chang, Barry Komm, Nichole Bayer Arnold, Murray Korc

Research output: Contribution to journalReview article

5 Scopus citations

Abstract

Differential display is an effective expression profiling tool which was first introduced in 1992. The original technique is discussed along with modifications that have been described over the last several years. A highly reproducible, semihigh-throughput differential display protocol used in our laboratories is described along with an example of its successful application using pancreatic cancer cells. In addition to the work performed in our laboratories, several examples of successful applications of differential display under a number of scenarios are reviewed. Differential display is one of several expression profiling technologies available and is compared with some of them. The future of differential display remains bright and is as applicable today as it was in 1992.

Original languageEnglish (US)
Pages (from-to)31-40
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume383
StatePublished - 2007

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Chang, K. C. N., Komm, B., Arnold, N. B., & Korc, M. (2007). The application of differential display as a gene profiling tool. Methods in molecular biology (Clifton, N.J.), 383, 31-40.