The application of differential display as a gene profiling tool.

Ken Chien Neng Chang, Barry Komm, Nichole Bayer Arnold, Murray Korc

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Differential display is an effective expression profiling tool which was first introduced in 1992. The original technique is discussed along with modifications that have been described over the last several years. A highly reproducible, semihigh-throughput differential display protocol used in our laboratories is described along with an example of its successful application using pancreatic cancer cells. In addition to the work performed in our laboratories, several examples of successful applications of differential display under a number of scenarios are reviewed. Differential display is one of several expression profiling technologies available and is compared with some of them. The future of differential display remains bright and is as applicable today as it was in 1992.

Original languageEnglish (US)
Pages (from-to)31-40
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume383
StatePublished - 2007
Externally publishedYes

Fingerprint

Pancreatic Neoplasms
Genes
Technology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Chang, K. C. N., Komm, B., Arnold, N. B., & Korc, M. (2007). The application of differential display as a gene profiling tool. Methods in molecular biology (Clifton, N.J.), 383, 31-40.

The application of differential display as a gene profiling tool. / Chang, Ken Chien Neng; Komm, Barry; Arnold, Nichole Bayer; Korc, Murray.

In: Methods in molecular biology (Clifton, N.J.), Vol. 383, 2007, p. 31-40.

Research output: Contribution to journalArticle

Chang, KCN, Komm, B, Arnold, NB & Korc, M 2007, 'The application of differential display as a gene profiling tool.', Methods in molecular biology (Clifton, N.J.), vol. 383, pp. 31-40.
Chang, Ken Chien Neng ; Komm, Barry ; Arnold, Nichole Bayer ; Korc, Murray. / The application of differential display as a gene profiling tool. In: Methods in molecular biology (Clifton, N.J.). 2007 ; Vol. 383. pp. 31-40.
@article{031bd9a65d434494a31e4dd06aa3f9cc,
title = "The application of differential display as a gene profiling tool.",
abstract = "Differential display is an effective expression profiling tool which was first introduced in 1992. The original technique is discussed along with modifications that have been described over the last several years. A highly reproducible, semihigh-throughput differential display protocol used in our laboratories is described along with an example of its successful application using pancreatic cancer cells. In addition to the work performed in our laboratories, several examples of successful applications of differential display under a number of scenarios are reviewed. Differential display is one of several expression profiling technologies available and is compared with some of them. The future of differential display remains bright and is as applicable today as it was in 1992.",
author = "Chang, {Ken Chien Neng} and Barry Komm and Arnold, {Nichole Bayer} and Murray Korc",
year = "2007",
language = "English (US)",
volume = "383",
pages = "31--40",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - The application of differential display as a gene profiling tool.

AU - Chang, Ken Chien Neng

AU - Komm, Barry

AU - Arnold, Nichole Bayer

AU - Korc, Murray

PY - 2007

Y1 - 2007

N2 - Differential display is an effective expression profiling tool which was first introduced in 1992. The original technique is discussed along with modifications that have been described over the last several years. A highly reproducible, semihigh-throughput differential display protocol used in our laboratories is described along with an example of its successful application using pancreatic cancer cells. In addition to the work performed in our laboratories, several examples of successful applications of differential display under a number of scenarios are reviewed. Differential display is one of several expression profiling technologies available and is compared with some of them. The future of differential display remains bright and is as applicable today as it was in 1992.

AB - Differential display is an effective expression profiling tool which was first introduced in 1992. The original technique is discussed along with modifications that have been described over the last several years. A highly reproducible, semihigh-throughput differential display protocol used in our laboratories is described along with an example of its successful application using pancreatic cancer cells. In addition to the work performed in our laboratories, several examples of successful applications of differential display under a number of scenarios are reviewed. Differential display is one of several expression profiling technologies available and is compared with some of them. The future of differential display remains bright and is as applicable today as it was in 1992.

UR - http://www.scopus.com/inward/record.url?scp=42449133874&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42449133874&partnerID=8YFLogxK

M3 - Article

VL - 383

SP - 31

EP - 40

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -