The asialoglycoprotein receptor is associated with a tyrosine kinase in HepG2 cells

Robert Fallon, Maria Danaher, Amit Saxena

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The asialoglycoprotein (ASGP) receptor undergoes constitutive endocytosis in HepG2 cells, which is regulated by tyrosine kinase activity (Fallon, R. J., Danaher, M., Saylors, R. L., and Saxena, A. (1994) J. Biol. Chem. 269, 11011-11017). In this study we show that the receptor copurifies with a tyrosine kinase activity, as defined by tyrosine phosphorylation of an exogenous substrate (reduced carboxyamidomethylated and maleylated lysozyme). Analysis of cells transfected with one subunit of the ASGP receptor showed that signals in the cytoplasmic domain of the H1 subunit are sufficient for receptor kinase association. In addition, receptor kinase association is not dependent on the single cytoplasmic tyrosine at position 5. Analysis of the components of anti-ASGP receptor immunoprecipitates revealed the presence of a 127-kDa protein (p127), which becomes phosphorylated on tyrosine upon addition of γ-[32P]ATP and which is capable of binding ATP. p127 was also demonstrated in anti-transferrin receptor immunoprecipitates but not in immunoprecipitates of a resident membrane protein, human HLA. In conclusion, these data demonstrate that the ASGP receptor, a protein that participates in constitutive, rapid endocytosis, is associated with a cellular tyrosine kinase.

Original languageEnglish (US)
Pages (from-to)26626-26629
Number of pages4
JournalJournal of Biological Chemistry
Volume269
Issue number43
StatePublished - Oct 28 1994
Externally publishedYes

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Asialoglycoprotein Receptor
Hep G2 Cells
Protein-Tyrosine Kinases
Tyrosine
Endocytosis
Phosphotransferases
Adenosine Triphosphate
Association reactions
Phosphorylation
Transferrin Receptors
Muramidase
Membrane Proteins
Proteins
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

The asialoglycoprotein receptor is associated with a tyrosine kinase in HepG2 cells. / Fallon, Robert; Danaher, Maria; Saxena, Amit.

In: Journal of Biological Chemistry, Vol. 269, No. 43, 28.10.1994, p. 26626-26629.

Research output: Contribution to journalArticle

Fallon, R, Danaher, M & Saxena, A 1994, 'The asialoglycoprotein receptor is associated with a tyrosine kinase in HepG2 cells', Journal of Biological Chemistry, vol. 269, no. 43, pp. 26626-26629.
Fallon, Robert ; Danaher, Maria ; Saxena, Amit. / The asialoglycoprotein receptor is associated with a tyrosine kinase in HepG2 cells. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 43. pp. 26626-26629.
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