The axolotl fibula as a model for the induction of regeneration across large segment defects in long bones of the extremities

Xiaoping Chen, Fengyu Song, Deepali Jhamb, Jiliang Li, Marco C. Bottino, Mathew J. Palakal, David L. Stocum

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We tested the ability of the axolotl (Ambystoma mexicanum) fibula to regenerate across segment defects of different size in the absence of intervention or after implant of a unique 8-braid pig small intestine submucosa (SIS) scaffold, with or without incorporated growth factor combinations or tissue protein extract. Fractures and defects of 10% and 20% of the total limb length regenerated well without any intervention, but 40% and 50% defects failed to regenerate after either simple removal of bone or implanting SIS scaffold alone. By contrast, scaffold soaked in the growth factor combination BMP-4/HGF or in protein extract of intact limb tissue promoted partial or extensive induction of cartilage and bone across 50% segment defects in 30%-33% of cases. These results show that BMP-4/HGF and intact tissue protein extract can promote the events required to induce cartilage and bone formation across a segment defect larger than critical size and that the long bones of axolotl limbs are an inexpensive model to screen soluble factors and natural and synthetic scaffolds for their efficacy in stimulating this process.

Original languageEnglish (US)
Article numbere0130819
JournalPLoS One
Volume10
Issue number6
DOIs
StatePublished - Jun 22 2015
Externally publishedYes

Fingerprint

Ambystoma mexicanum
fibula
Fibula
Regeneration
Bone
Tissue Extracts
Extremities
Scaffolds
bones
limbs (animal)
Bone and Bones
cartilage
Defects
growth factors
Small Intestine
Cartilage
small intestine
Intercellular Signaling Peptides and Proteins
extracts
limb bones

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Chen, X., Song, F., Jhamb, D., Li, J., Bottino, M. C., Palakal, M. J., & Stocum, D. L. (2015). The axolotl fibula as a model for the induction of regeneration across large segment defects in long bones of the extremities. PLoS One, 10(6), [e0130819]. https://doi.org/10.1371/journal.pone.0130819

The axolotl fibula as a model for the induction of regeneration across large segment defects in long bones of the extremities. / Chen, Xiaoping; Song, Fengyu; Jhamb, Deepali; Li, Jiliang; Bottino, Marco C.; Palakal, Mathew J.; Stocum, David L.

In: PLoS One, Vol. 10, No. 6, e0130819, 22.06.2015.

Research output: Contribution to journalArticle

Chen, Xiaoping ; Song, Fengyu ; Jhamb, Deepali ; Li, Jiliang ; Bottino, Marco C. ; Palakal, Mathew J. ; Stocum, David L. / The axolotl fibula as a model for the induction of regeneration across large segment defects in long bones of the extremities. In: PLoS One. 2015 ; Vol. 10, No. 6.
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