The beneficial effects of dichloroacetate in acute limb ischemia

Timothy A. Platz, Jeffrey S. Wilson, Jeffrey A. Kline, Greg Rushing, Jaime L. Parker, Erin M. Moore, Fredrick N. Southern

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Abstract

Objective: The purpose of this study was to determine the effects of dichloroacetate (DCA) in acute limb ischemia. Methods: Anterior tibialis muscle samples of DCA-treated and control animals (Sprague Dawley rats) were collected and assayed for pyruvate dehydrogenase activity, lactate, adenosine triphosphate, and creatine phosphate using spectrophotometry. A physiograph was used to measure fatigability. In an ischemia/reperfusion model using New Zealand rabbits, serum lactate and end-tidal CO2 were compared. Skeletal muscle was evaluated microscopically for muscle necrosis. Results: DCA administration resulted in a 50% increase in pyruvate dehydrogenase activity (p = 0.025), reversal of the increase in lactate levels seen during acute limb ischemia (p = 0.41), a significant increase in the time to skeletal muscle fatigue (p = 0.05), a trend toward increased adenosine triphosphate (p = 0.07), and a significant increase in creatine phosphate (p < 0.02). DCA treatment resulted in a decrease in serum lactate (p < 0.01) and end-tidal CO 2 (p < 0.001). Conclusion: In acute limb ischemia and reperfusion, DCA administration provides metabolic protection to skeletal muscle.

Original languageEnglish (US)
Pages (from-to)628-633
Number of pages6
JournalMilitary medicine
Volume172
Issue number6
DOIs
StatePublished - Jun 2007

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ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

Platz, T. A., Wilson, J. S., Kline, J. A., Rushing, G., Parker, J. L., Moore, E. M., & Southern, F. N. (2007). The beneficial effects of dichloroacetate in acute limb ischemia. Military medicine, 172(6), 628-633. https://doi.org/10.7205/MILMED.172.6.628