The beneficial effects of dichloroacetate in acute limb ischemia

Timothy A. Platz, Jeffrey S. Wilson, Jeffrey Kline, Greg Rushing, Jaime L. Parker, Erin M. Moore, Fredrick N. Southern

Research output: Contribution to journalArticle

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Abstract

Objective: The purpose of this study was to determine the effects of dichloroacetate (DCA) in acute limb ischemia. Methods: Anterior tibialis muscle samples of DCA-treated and control animals (Sprague Dawley rats) were collected and assayed for pyruvate dehydrogenase activity, lactate, adenosine triphosphate, and creatine phosphate using spectrophotometry. A physiograph was used to measure fatigability. In an ischemia/reperfusion model using New Zealand rabbits, serum lactate and end-tidal CO2 were compared. Skeletal muscle was evaluated microscopically for muscle necrosis. Results: DCA administration resulted in a 50% increase in pyruvate dehydrogenase activity (p = 0.025), reversal of the increase in lactate levels seen during acute limb ischemia (p = 0.41), a significant increase in the time to skeletal muscle fatigue (p = 0.05), a trend toward increased adenosine triphosphate (p = 0.07), and a significant increase in creatine phosphate (p <0.02). DCA treatment resulted in a decrease in serum lactate (p <0.01) and end-tidal CO 2 (p <0.001). Conclusion: In acute limb ischemia and reperfusion, DCA administration provides metabolic protection to skeletal muscle.

Original languageEnglish (US)
Pages (from-to)628-633
Number of pages6
JournalMilitary Medicine
Volume172
Issue number6
StatePublished - Jun 2007
Externally publishedYes

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Ischemia
Extremities
Lactic Acid
Skeletal Muscle
Phosphocreatine
Pyruvic Acid
Reperfusion
Adenosine Triphosphate
Muscles
Muscle Fatigue
Adenine Nucleotides
Spectrophotometry
Carbon Monoxide
Serum
L-Lactate Dehydrogenase
Sprague Dawley Rats
Oxidoreductases
Necrosis
Rabbits

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

Platz, T. A., Wilson, J. S., Kline, J., Rushing, G., Parker, J. L., Moore, E. M., & Southern, F. N. (2007). The beneficial effects of dichloroacetate in acute limb ischemia. Military Medicine, 172(6), 628-633.

The beneficial effects of dichloroacetate in acute limb ischemia. / Platz, Timothy A.; Wilson, Jeffrey S.; Kline, Jeffrey; Rushing, Greg; Parker, Jaime L.; Moore, Erin M.; Southern, Fredrick N.

In: Military Medicine, Vol. 172, No. 6, 06.2007, p. 628-633.

Research output: Contribution to journalArticle

Platz, TA, Wilson, JS, Kline, J, Rushing, G, Parker, JL, Moore, EM & Southern, FN 2007, 'The beneficial effects of dichloroacetate in acute limb ischemia', Military Medicine, vol. 172, no. 6, pp. 628-633.
Platz TA, Wilson JS, Kline J, Rushing G, Parker JL, Moore EM et al. The beneficial effects of dichloroacetate in acute limb ischemia. Military Medicine. 2007 Jun;172(6):628-633.
Platz, Timothy A. ; Wilson, Jeffrey S. ; Kline, Jeffrey ; Rushing, Greg ; Parker, Jaime L. ; Moore, Erin M. ; Southern, Fredrick N. / The beneficial effects of dichloroacetate in acute limb ischemia. In: Military Medicine. 2007 ; Vol. 172, No. 6. pp. 628-633.
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