The bovine papillomavirus type 1 E6 oncoprotein sensitizes cells to tumor necrosis factor alpha-induced apoptosis

Lisa Rapp, Yun Liu, Yihui Hong, Elliot J. Androphy, Jason J. Chen

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Expression of viral proteins may result in susceptibility of cells to the cytotoxic effect of Tumor Necrosis Factor Alpha (TNF). While murine C127 cells containing the bovine papillomavirus type 1 (BPV-1) genome were reported to exhibit increased TNF sensitivity, the gene(s) responsible was not identified. The BPV-1 E6 oncoprotein induces tumorigenic transformation of murine C127 cells and stimulates transcription when targeted to a promoter. BPV-1 E6 was introduced into C127 cells (PBE6) by retroviral infection and stable clones were isolated. These cells showed increased apoptosis in response to TNF, as measured by several criteria. TNF-induced apoptosis in PBE6 cells was accompanied by increased release of arachidonic acid, indicating that phospholipase A2 was activated. We also provide evidence that BPV-1 E6 mediated-sensitization of cells to TNF-induced apoptosis can ocur in the absence of p53.

Original languageEnglish (US)
Pages (from-to)607-615
Number of pages9
JournalOncogene
Volume18
Issue number3
DOIs
StatePublished - Jan 21 1999

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Keywords

  • Apoptosis
  • Arachidonic acid
  • BPV-1 E6
  • Cytolysis
  • TNF
  • p53

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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