The BPV-1 E2 DNA-contact helix cysteine is required for transcriptional activation but not replication in mammalian cells

Martha J. Grossel, James Barsoum, S. S. Prakash, Elliot Androphy

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The papillomavirus E2 protein contains an amino-terminal region thought necessary and sufficient to support transcriptional activation and a carboxy-terminal region shown to direct sequence-specific DNA binding and dimerization. A cysteine residue in the center of the E2 DNA recognition helix is highly conserved among papillomavirus E2 proteins. Mutations of this cysteine in bovine papillomavirus type 1 E2 to serine and glycine resulted in proteins which failed to activate E2-dependent promoters in mammalian cells. These E2 mutants were DNA-binding competent, dimeric, and nuclear. When fused to the VP16 transactivation domain, C-terminal regions of E2 containing the mutations at 340 supported transcriptional activation, indicating that the heterologous trans-activation domain did not require cysteine in the DNA-binding helix as did the full-length E2 transactivating protein. Although cysteine-340 was required for transcriptional activation it was not required for DNA replication in vivo. Together, these results suggest that the E2 DNA-binding domain may directly contribute to functions of transcriptional activation previously thought limited to the N-terminal domain.

Original languageEnglish (US)
Pages (from-to)301-310
Number of pages10
JournalVirology
Volume217
Issue number1
DOIs
StatePublished - Mar 1 1996
Externally publishedYes

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Transcriptional Activation
Cysteine
DNA
Proteins
Bovine papillomavirus 1
Mutation
Dimerization
DNA Replication
Glycine
Serine

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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The BPV-1 E2 DNA-contact helix cysteine is required for transcriptional activation but not replication in mammalian cells. / Grossel, Martha J.; Barsoum, James; Prakash, S. S.; Androphy, Elliot.

In: Virology, Vol. 217, No. 1, 01.03.1996, p. 301-310.

Research output: Contribution to journalArticle

Grossel, Martha J. ; Barsoum, James ; Prakash, S. S. ; Androphy, Elliot. / The BPV-1 E2 DNA-contact helix cysteine is required for transcriptional activation but not replication in mammalian cells. In: Virology. 1996 ; Vol. 217, No. 1. pp. 301-310.
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