The characterization of versican and its message in human articular cartilage and intervertebral disc

Robert Sztrolovics, Judy Grover, Gabriella Cs-Szabo, Shui Liang Shi, Yiping Zhang, John S. Mort, Peter J. Roughley

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Splicing variation of the versican message and size heterogeneity of the versican core protein were analyzed in human articular cartilage and intervertebral disc. Splicing variation of the message was studied by PCR analysis to detect the presence or absence of exons 7 and 8, which encode large chondroitin sulfate attachment regions. At all ages in normal cartilage from the third trimester fetus to the mature adult, the presence of the versican isoform possessing exon 8 but not exon 7 (V1) could be readily detected. The message isoforms possessing neither exon 7 nor 8 (V3) or both exons 7 and 8 (V0) were only detectable in the fetus, and the isoform possessing only exon 7 (V2) was never detected. In osteoarthritic cartilage and in adult intervertebral disc the versican message pattern was the same as that observed in the normal adult with only the isoform possessing exon 8 being detected. Core protein heterogeneity was studied by immunoblotting following enzymic removal of the glycosaminoglycan chains from the proteoglycan, using an antibody recognizing the globular G1 region of versican. All articular cartilage extracts from the fetus to the mature adult contained multiple core protein sizes of greater than 200 kDa. The adult cartilage extracts tended to have an increased proportion of the smaller sized core proteins and osteoarthritic cartilage possessed similar core protein sizes to the normal adult. In contrast, intervertebral disc at all post-natal ages showed a greater range of size heterogeneity with a prominent component of about 50 kDa. The abundance of this component increased if the samples were treated with keratanase prior to analysis, suggesting that the G1 region of versican in disc can be substituted with keratan sulfate. The increased presence of versican in the disc relative to articular cartilage may suggest a more pronounced functional role for this proteoglycan, particularly in the nucleus pulposus.

Original languageEnglish (US)
Pages (from-to)257-266
Number of pages10
JournalJournal of Orthopaedic Research
Volume20
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

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Versicans
Intervertebral Disc
Articular Cartilage
Exons
Cartilage
Protein Isoforms
Fetus
keratan-sulfate endo-1,4-beta-galactosidase
Proteoglycans
Proteins
Keratan Sulfate
Chondroitin Sulfates
Third Pregnancy Trimester
Glycosaminoglycans
Immunoblotting
Polymerase Chain Reaction
Antibodies

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Sztrolovics, R., Grover, J., Cs-Szabo, G., Shi, S. L., Zhang, Y., Mort, J. S., & Roughley, P. J. (2002). The characterization of versican and its message in human articular cartilage and intervertebral disc. Journal of Orthopaedic Research, 20(2), 257-266. https://doi.org/10.1016/S0736-0266(01)00110-3

The characterization of versican and its message in human articular cartilage and intervertebral disc. / Sztrolovics, Robert; Grover, Judy; Cs-Szabo, Gabriella; Shi, Shui Liang; Zhang, Yiping; Mort, John S.; Roughley, Peter J.

In: Journal of Orthopaedic Research, Vol. 20, No. 2, 2002, p. 257-266.

Research output: Contribution to journalArticle

Sztrolovics, R, Grover, J, Cs-Szabo, G, Shi, SL, Zhang, Y, Mort, JS & Roughley, PJ 2002, 'The characterization of versican and its message in human articular cartilage and intervertebral disc', Journal of Orthopaedic Research, vol. 20, no. 2, pp. 257-266. https://doi.org/10.1016/S0736-0266(01)00110-3
Sztrolovics, Robert ; Grover, Judy ; Cs-Szabo, Gabriella ; Shi, Shui Liang ; Zhang, Yiping ; Mort, John S. ; Roughley, Peter J. / The characterization of versican and its message in human articular cartilage and intervertebral disc. In: Journal of Orthopaedic Research. 2002 ; Vol. 20, No. 2. pp. 257-266.
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