The CHD3 remodeler PICKLE promotes trimethylation of histone H3 lysine 27

Heng Zhang, Stanley Dean Rider, James T. Henderson, Matthew Fountain, King Chuang, Vasundhara Kandachar, Alexis Simons, Howard J. Edenberg, Jeanne Romero-Severson, William M. Muir, Joe Ogas

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

CHD3 proteins are ATP-dependent chromatin remodelers that contribute to repression of developmentally regulated genes in both animal and plant systems. In animals, this repression has been linked to a multiple subunit complex, Mi-2/NuRD, whose constituents include a CHD3 protein, a histone deacetylase, and a methyl-CpG-binding domain protein. In Arabidopsis, PICKLE (PKL) codes for a CHD3 protein that acts during germination to repress expression of seed-associated genes. Repression of seed-associated traits is promoted in pkl seedlings by the plant growth regulator gibberellin (GA). We undertook a microarray analysis to determine how PKL and GA act to promote the transition from seed to seedling. We found that PKL and GA act in separate pathways to repress expression of seed-specific genes. Comparison of genomic datasets revealed that PKL-dependent genes are enriched for trimethylation of histone H3 lysine 27 (H3K27me3), a repressive epigenetic mark. Chromatin immunoprecipitation studies demonstrate that PKL promotes H3K27me3 in both germinating seedlings and in adult plants but do not identify a connection between PKL-dependent expression and acetylation levels. Taken together, our analyses illuminate a new pathway by which CHD3 remodelers contribute to repression in eukaryotes.

Original languageEnglish (US)
Pages (from-to)22637-22648
Number of pages12
JournalJournal of Biological Chemistry
Volume283
Issue number33
DOIs
StatePublished - Aug 15 2008

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Zhang, H., Rider, S. D., Henderson, J. T., Fountain, M., Chuang, K., Kandachar, V., Simons, A., Edenberg, H. J., Romero-Severson, J., Muir, W. M., & Ogas, J. (2008). The CHD3 remodeler PICKLE promotes trimethylation of histone H3 lysine 27. Journal of Biological Chemistry, 283(33), 22637-22648. https://doi.org/10.1074/jbc.M802129200