The chicken frizzle feather is due to an α-Keratin (KRT75) mutation that causes a defective rachis

Chen Siang Ng, Ping Wu, John Foley, Anne Foley, Merry Lynn McDonald, Wen Tau Juan, Chih Jen Huang, Yu Ting Lai, Wen Sui Lo, Chih Feng Chen, Suzanne M. Leal, Huanmin Zhang, Randall B. Widelitz, Pragna I. Patel, Wen Hsiung Li, Cheng Ming Chuong

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Feathers have complex forms and are an excellent model to study the development and evolution of morphologies. Existing chicken feather mutants are especially useful for identifying genetic determinants of feather formation. This study focused on the gene F, underlying the frizzle feather trait that has a characteristic curled feather rachis and barbs in domestic chickens. Our developmental biology studies identified defects in feather medulla formation, and physical studies revealed that the frizzle feather curls in a stepwise manner. The frizzle gene is transmitted in an autosomal incomplete dominant mode. A whole-genome linkage scan of five pedigrees with 2678 SNPs revealed association of the frizzle locus with a keratin gene-enriched region within the linkage group E22C19W28_E50C23. Sequence analyses of the keratin gene cluster identified a 69 bp in-frame deletion in a conserved region of KRT75, an α-keratin gene. Retroviral-mediated expression of the mutated F cDNA in the wild-type rectrix qualitatively changed the bending of the rachis with some features of frizzle feathers including irregular kinks, severe bending near their distal ends, and substantially higher variations among samples in comparison to normal feathers. These results confirmed KRT75 as the F gene. This study demonstrates the potential of our approach for identifying genetic determinants of feather forms.

Original languageEnglish (US)
Article numbere1002748
JournalPLoS Genetics
Volume8
Issue number7
DOIs
StatePublished - Jul 1 2012

Fingerprint

Feathers
keratin
feather
Keratins
feathers
Chickens
mutation
chickens
Mutation
gene
Genes
genes
developmental biology
Developmental Biology
Pedigree
Multigene Family
linkage groups
multigene family
pedigree
Single Nucleotide Polymorphism

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

The chicken frizzle feather is due to an α-Keratin (KRT75) mutation that causes a defective rachis. / Ng, Chen Siang; Wu, Ping; Foley, John; Foley, Anne; McDonald, Merry Lynn; Juan, Wen Tau; Huang, Chih Jen; Lai, Yu Ting; Lo, Wen Sui; Chen, Chih Feng; Leal, Suzanne M.; Zhang, Huanmin; Widelitz, Randall B.; Patel, Pragna I.; Li, Wen Hsiung; Chuong, Cheng Ming.

In: PLoS Genetics, Vol. 8, No. 7, e1002748, 01.07.2012.

Research output: Contribution to journalArticle

Ng, CS, Wu, P, Foley, J, Foley, A, McDonald, ML, Juan, WT, Huang, CJ, Lai, YT, Lo, WS, Chen, CF, Leal, SM, Zhang, H, Widelitz, RB, Patel, PI, Li, WH & Chuong, CM 2012, 'The chicken frizzle feather is due to an α-Keratin (KRT75) mutation that causes a defective rachis', PLoS Genetics, vol. 8, no. 7, e1002748. https://doi.org/10.1371/journal.pgen.1002748
Ng, Chen Siang ; Wu, Ping ; Foley, John ; Foley, Anne ; McDonald, Merry Lynn ; Juan, Wen Tau ; Huang, Chih Jen ; Lai, Yu Ting ; Lo, Wen Sui ; Chen, Chih Feng ; Leal, Suzanne M. ; Zhang, Huanmin ; Widelitz, Randall B. ; Patel, Pragna I. ; Li, Wen Hsiung ; Chuong, Cheng Ming. / The chicken frizzle feather is due to an α-Keratin (KRT75) mutation that causes a defective rachis. In: PLoS Genetics. 2012 ; Vol. 8, No. 7.
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