The combination of platelet‐derived growth factor‐BB and insulin‐like growth factor‐I stimulates bone repair in adult Yucatan miniature pigs

Samuel E. Lynch, Stephen Trippel, Richard D. Finkelman, Rafael A. Hernandez, Christopher P. Kiritsy, Harry N. Antoniades

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The combination of insulin‐like growth factor‐I and platelet‐derived growth factor‐BB has previously been shown to stimulate healing of soft tissue wounds and the formation of bone and ligament around teeth. The purpose of the present study was to evaluate the effects of platelet‐derived growth factor‐BB and insulin‐like growth factor‐I individually and in combination on the healing of osseous wounds. Four standardized cortical wounds were created in each tibia of 11 adult Yucatan miniature pigs. The wounds in one tibia per animal were treated with either purified recombinant human insulin‐like growth factor‐I, platelet‐derived growth factor‐BB, or both in a methylcellulose gel. The wounds in each contralateral tibia received placebo gel alone. Coded serial sections of each wound were evaluated by computer‐aided histomorphometry 21 days after surgery. The area and perimeter of the newly formed mineralized callus, the thickness of the total callus, and the percentage of mineralized tissue within the callus were significantly increased compared with the values of matched controls only in wounds treated with a combination of insulin‐like growth factor‐I and platelet‐derived growth factor‐BB. No significant differences in the measured parameters of callus formation were found in wounds treated with either insulin‐like growth factor‐I or platelet‐derived growth factor‐BB alone. Cartilage was present only in sites treated with insulin‐like growth factor‐I alone. These results suggest that the combination of platelet‐derived growth factor‐BB and insulin‐like growth factor‐I stimulates bone formation in wounds in long bones of adult animals and that these growth factors act via different pathways during the repair process.

Original languageEnglish (US)
Pages (from-to)182-190
Number of pages9
JournalWound Repair and Regeneration
Volume2
Issue number3
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

Swine
Bone and Bones
Growth
Wounds and Injuries
Bony Callus
Tibia
Osteogenesis
Gels
Methylcellulose
Ambulatory Surgical Procedures
Ligaments
Wound Healing
Cartilage
Intercellular Signaling Peptides and Proteins
Tooth
Placebos

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

The combination of platelet‐derived growth factor‐BB and insulin‐like growth factor‐I stimulates bone repair in adult Yucatan miniature pigs. / Lynch, Samuel E.; Trippel, Stephen; Finkelman, Richard D.; Hernandez, Rafael A.; Kiritsy, Christopher P.; Antoniades, Harry N.

In: Wound Repair and Regeneration, Vol. 2, No. 3, 1994, p. 182-190.

Research output: Contribution to journalArticle

Lynch, Samuel E. ; Trippel, Stephen ; Finkelman, Richard D. ; Hernandez, Rafael A. ; Kiritsy, Christopher P. ; Antoniades, Harry N. / The combination of platelet‐derived growth factor‐BB and insulin‐like growth factor‐I stimulates bone repair in adult Yucatan miniature pigs. In: Wound Repair and Regeneration. 1994 ; Vol. 2, No. 3. pp. 182-190.
@article{34aed7b1e3064714a20497354691f84e,
title = "The combination of platelet‐derived growth factor‐BB and insulin‐like growth factor‐I stimulates bone repair in adult Yucatan miniature pigs",
abstract = "The combination of insulin‐like growth factor‐I and platelet‐derived growth factor‐BB has previously been shown to stimulate healing of soft tissue wounds and the formation of bone and ligament around teeth. The purpose of the present study was to evaluate the effects of platelet‐derived growth factor‐BB and insulin‐like growth factor‐I individually and in combination on the healing of osseous wounds. Four standardized cortical wounds were created in each tibia of 11 adult Yucatan miniature pigs. The wounds in one tibia per animal were treated with either purified recombinant human insulin‐like growth factor‐I, platelet‐derived growth factor‐BB, or both in a methylcellulose gel. The wounds in each contralateral tibia received placebo gel alone. Coded serial sections of each wound were evaluated by computer‐aided histomorphometry 21 days after surgery. The area and perimeter of the newly formed mineralized callus, the thickness of the total callus, and the percentage of mineralized tissue within the callus were significantly increased compared with the values of matched controls only in wounds treated with a combination of insulin‐like growth factor‐I and platelet‐derived growth factor‐BB. No significant differences in the measured parameters of callus formation were found in wounds treated with either insulin‐like growth factor‐I or platelet‐derived growth factor‐BB alone. Cartilage was present only in sites treated with insulin‐like growth factor‐I alone. These results suggest that the combination of platelet‐derived growth factor‐BB and insulin‐like growth factor‐I stimulates bone formation in wounds in long bones of adult animals and that these growth factors act via different pathways during the repair process.",
author = "Lynch, {Samuel E.} and Stephen Trippel and Finkelman, {Richard D.} and Hernandez, {Rafael A.} and Kiritsy, {Christopher P.} and Antoniades, {Harry N.}",
year = "1994",
doi = "10.1046/j.1524-475X.1994.20308.x",
language = "English (US)",
volume = "2",
pages = "182--190",
journal = "Wound Repair and Regeneration",
issn = "1067-1927",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - The combination of platelet‐derived growth factor‐BB and insulin‐like growth factor‐I stimulates bone repair in adult Yucatan miniature pigs

AU - Lynch, Samuel E.

AU - Trippel, Stephen

AU - Finkelman, Richard D.

AU - Hernandez, Rafael A.

AU - Kiritsy, Christopher P.

AU - Antoniades, Harry N.

PY - 1994

Y1 - 1994

N2 - The combination of insulin‐like growth factor‐I and platelet‐derived growth factor‐BB has previously been shown to stimulate healing of soft tissue wounds and the formation of bone and ligament around teeth. The purpose of the present study was to evaluate the effects of platelet‐derived growth factor‐BB and insulin‐like growth factor‐I individually and in combination on the healing of osseous wounds. Four standardized cortical wounds were created in each tibia of 11 adult Yucatan miniature pigs. The wounds in one tibia per animal were treated with either purified recombinant human insulin‐like growth factor‐I, platelet‐derived growth factor‐BB, or both in a methylcellulose gel. The wounds in each contralateral tibia received placebo gel alone. Coded serial sections of each wound were evaluated by computer‐aided histomorphometry 21 days after surgery. The area and perimeter of the newly formed mineralized callus, the thickness of the total callus, and the percentage of mineralized tissue within the callus were significantly increased compared with the values of matched controls only in wounds treated with a combination of insulin‐like growth factor‐I and platelet‐derived growth factor‐BB. No significant differences in the measured parameters of callus formation were found in wounds treated with either insulin‐like growth factor‐I or platelet‐derived growth factor‐BB alone. Cartilage was present only in sites treated with insulin‐like growth factor‐I alone. These results suggest that the combination of platelet‐derived growth factor‐BB and insulin‐like growth factor‐I stimulates bone formation in wounds in long bones of adult animals and that these growth factors act via different pathways during the repair process.

AB - The combination of insulin‐like growth factor‐I and platelet‐derived growth factor‐BB has previously been shown to stimulate healing of soft tissue wounds and the formation of bone and ligament around teeth. The purpose of the present study was to evaluate the effects of platelet‐derived growth factor‐BB and insulin‐like growth factor‐I individually and in combination on the healing of osseous wounds. Four standardized cortical wounds were created in each tibia of 11 adult Yucatan miniature pigs. The wounds in one tibia per animal were treated with either purified recombinant human insulin‐like growth factor‐I, platelet‐derived growth factor‐BB, or both in a methylcellulose gel. The wounds in each contralateral tibia received placebo gel alone. Coded serial sections of each wound were evaluated by computer‐aided histomorphometry 21 days after surgery. The area and perimeter of the newly formed mineralized callus, the thickness of the total callus, and the percentage of mineralized tissue within the callus were significantly increased compared with the values of matched controls only in wounds treated with a combination of insulin‐like growth factor‐I and platelet‐derived growth factor‐BB. No significant differences in the measured parameters of callus formation were found in wounds treated with either insulin‐like growth factor‐I or platelet‐derived growth factor‐BB alone. Cartilage was present only in sites treated with insulin‐like growth factor‐I alone. These results suggest that the combination of platelet‐derived growth factor‐BB and insulin‐like growth factor‐I stimulates bone formation in wounds in long bones of adult animals and that these growth factors act via different pathways during the repair process.

UR - http://www.scopus.com/inward/record.url?scp=84989012431&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84989012431&partnerID=8YFLogxK

U2 - 10.1046/j.1524-475X.1994.20308.x

DO - 10.1046/j.1524-475X.1994.20308.x

M3 - Article

VL - 2

SP - 182

EP - 190

JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

SN - 1067-1927

IS - 3

ER -