The conformational basis of energy transduction in membrane systems. V. Measurement of configurational changes by light scattering

Robert Harris, Mary Ann Asbell, Junpei Asai, Walter W. Jolly, David E. Green

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Changes in the energy state of the cristal membrane of beef heart mitochondria can be followed continuously by light-scattering measurements at 90 ° to the incident beam. Only two of the three configurational transitions established by electron microscopy can be directly measured by the light-scattering technique. The correlation between the configurational changes deduced from the interpretation of electron micrographs, and the configurational changes implicit in the light-scattering measurements has been tested in a wide spectrum of experiments and found to hold in all cases. It has been concluded that the electron microscopic and light-scattering techniques both measure configurational change in the cristal membrane. The transition of cristae from the aggregated to the orthodox modality, and the oscillation of mitochondria between the energized uptake of K+ and swelling, and the de-energized release of K+ and shrinking, are phenomena which are readily analyzed by the method of light scattering in combination with that of electron microscopy.

Original languageEnglish (US)
Pages (from-to)545-560
Number of pages16
JournalArchives of Biochemistry and Biophysics
Volume132
Issue number2
StatePublished - Jul 1969
Externally publishedYes

Fingerprint

Light scattering
Membranes
Light
Mitochondria
Electron microscopy
Electron Microscopy
Electrons
Heart Mitochondria
Beef
Electron energy levels
Swelling
Experiments

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

The conformational basis of energy transduction in membrane systems. V. Measurement of configurational changes by light scattering. / Harris, Robert; Asbell, Mary Ann; Asai, Junpei; Jolly, Walter W.; Green, David E.

In: Archives of Biochemistry and Biophysics, Vol. 132, No. 2, 07.1969, p. 545-560.

Research output: Contribution to journalArticle

@article{f0cbe71cd5854dfa926167043996c573,
title = "The conformational basis of energy transduction in membrane systems. V. Measurement of configurational changes by light scattering",
abstract = "Changes in the energy state of the cristal membrane of beef heart mitochondria can be followed continuously by light-scattering measurements at 90 ° to the incident beam. Only two of the three configurational transitions established by electron microscopy can be directly measured by the light-scattering technique. The correlation between the configurational changes deduced from the interpretation of electron micrographs, and the configurational changes implicit in the light-scattering measurements has been tested in a wide spectrum of experiments and found to hold in all cases. It has been concluded that the electron microscopic and light-scattering techniques both measure configurational change in the cristal membrane. The transition of cristae from the aggregated to the orthodox modality, and the oscillation of mitochondria between the energized uptake of K+ and swelling, and the de-energized release of K+ and shrinking, are phenomena which are readily analyzed by the method of light scattering in combination with that of electron microscopy.",
author = "Robert Harris and Asbell, {Mary Ann} and Junpei Asai and Jolly, {Walter W.} and Green, {David E.}",
year = "1969",
month = "7",
language = "English (US)",
volume = "132",
pages = "545--560",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The conformational basis of energy transduction in membrane systems. V. Measurement of configurational changes by light scattering

AU - Harris, Robert

AU - Asbell, Mary Ann

AU - Asai, Junpei

AU - Jolly, Walter W.

AU - Green, David E.

PY - 1969/7

Y1 - 1969/7

N2 - Changes in the energy state of the cristal membrane of beef heart mitochondria can be followed continuously by light-scattering measurements at 90 ° to the incident beam. Only two of the three configurational transitions established by electron microscopy can be directly measured by the light-scattering technique. The correlation between the configurational changes deduced from the interpretation of electron micrographs, and the configurational changes implicit in the light-scattering measurements has been tested in a wide spectrum of experiments and found to hold in all cases. It has been concluded that the electron microscopic and light-scattering techniques both measure configurational change in the cristal membrane. The transition of cristae from the aggregated to the orthodox modality, and the oscillation of mitochondria between the energized uptake of K+ and swelling, and the de-energized release of K+ and shrinking, are phenomena which are readily analyzed by the method of light scattering in combination with that of electron microscopy.

AB - Changes in the energy state of the cristal membrane of beef heart mitochondria can be followed continuously by light-scattering measurements at 90 ° to the incident beam. Only two of the three configurational transitions established by electron microscopy can be directly measured by the light-scattering technique. The correlation between the configurational changes deduced from the interpretation of electron micrographs, and the configurational changes implicit in the light-scattering measurements has been tested in a wide spectrum of experiments and found to hold in all cases. It has been concluded that the electron microscopic and light-scattering techniques both measure configurational change in the cristal membrane. The transition of cristae from the aggregated to the orthodox modality, and the oscillation of mitochondria between the energized uptake of K+ and swelling, and the de-energized release of K+ and shrinking, are phenomena which are readily analyzed by the method of light scattering in combination with that of electron microscopy.

UR - http://www.scopus.com/inward/record.url?scp=0014545351&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0014545351&partnerID=8YFLogxK

M3 - Article

VL - 132

SP - 545

EP - 560

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 2

ER -