The creation of an in vitro adipose tissue that contains a vascular-adipocyte complex

J. Michael Sorrell, Marilyn A. Baber, Dmitry Traktuev, Keith L. March, Arnold I. Caplan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

An increased demand for soft-tissue substitutes has impelled the development of an in vitro adipose tissue. Ideally, such a tissue should contain a vascular network that can deliver blood throughout the construct following its engraftment. This study describes the in vitro fabrication of a pre-vascularized adipose tissue entirely using a self-assembly approach. Adult human adipose stromal cells (ASCs) provided the foundation for this construct. These cells were cultured at high density in the presence of elevated levels of ascorbate prior to adipocytic induction. Vascular support cells consisting of dermal fibroblasts, mixtures of adipose stromal cells and bone marrow mesenchymal stem cells (MSCs) were introduced to sustain an extensive vascular network formed by human umbilical vein endothelial cells (HUVECs). MSCs were introduced to serve as perivascular cells. The resulting construct contained a vascular-adipose tissue continuum that was held together by basement membrane molecules. This construct contains multiple cell types that are typically found in adipose tissue: adipocytes, pre-adipocytes, stem cells, fibroblasts, vascular cells, and perivascular support cells. As such, these constructs can be employed both for in vitro studies to assay cellular interactions between vasculature and other components of adipose tissue. Further, they can also be engrafted into athymic hosts to study vascular and adipocyte stability.

Original languageEnglish
Pages (from-to)9667-9676
Number of pages10
JournalBiomaterials
Volume32
Issue number36
DOIs
StatePublished - Dec 2011

Fingerprint

Adipocytes
Blood Vessels
Adipose Tissue
Tissue
Mesenchymal Stromal Cells
Stem cells
Fibroblasts
Human Umbilical Vein Endothelial Cells
Stromal Cells
Basement Membrane
Endothelial cells
In Vitro Techniques
Cultured Cells
Self assembly
Stem Cells
Assays
Bone
Blood
Skin
Fabrication

Keywords

  • Adipose tissue engineering
  • Angiogenesis
  • Co-culture
  • ECM
  • Fibroblast
  • Mesenchymal stem cell

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

The creation of an in vitro adipose tissue that contains a vascular-adipocyte complex. / Michael Sorrell, J.; Baber, Marilyn A.; Traktuev, Dmitry; March, Keith L.; Caplan, Arnold I.

In: Biomaterials, Vol. 32, No. 36, 12.2011, p. 9667-9676.

Research output: Contribution to journalArticle

Michael Sorrell, J. ; Baber, Marilyn A. ; Traktuev, Dmitry ; March, Keith L. ; Caplan, Arnold I. / The creation of an in vitro adipose tissue that contains a vascular-adipocyte complex. In: Biomaterials. 2011 ; Vol. 32, No. 36. pp. 9667-9676.
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