The DDN catalytic motif is required for Metnase functions in non-homologous end joining (NHEJ) repair and replication restart

Hyun Suk Kim, Qiujia Chen, Sung Kyung Kim, Jac A. Nickoloff, Robert Hromas, Millie Georgiadis, Suk-Hee Lee

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Metnase, a transposase-containing DNA repair protein, retains DNA cleavage activity with a DDN motif. Results: Substitution with the ancestral transposase DDD/DDE catalytic motif results in a decrease in ssDNA binding and ss-overhang cleavage activities. Conclusion: The DDN motif is required for Metnase DNA repair activities. Significance: Understanding the requirements for catalytic activity provides insights on how Metnase functions as a DNA repair protein.

Original languageEnglish
Pages (from-to)10930-10938
Number of pages9
JournalJournal of Biological Chemistry
Volume289
Issue number15
DOIs
StatePublished - Apr 11 2014

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Joining
DNA Repair
Transposases
Repair
DNA
Dichlorodiphenyl Dichloroethylene
Dichlorodiphenyldichloroethane
DNA Cleavage
Proteins
Catalyst activity
Substitution reactions

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

The DDN catalytic motif is required for Metnase functions in non-homologous end joining (NHEJ) repair and replication restart. / Kim, Hyun Suk; Chen, Qiujia; Kim, Sung Kyung; Nickoloff, Jac A.; Hromas, Robert; Georgiadis, Millie; Lee, Suk-Hee.

In: Journal of Biological Chemistry, Vol. 289, No. 15, 11.04.2014, p. 10930-10938.

Research output: Contribution to journalArticle

Kim, Hyun Suk ; Chen, Qiujia ; Kim, Sung Kyung ; Nickoloff, Jac A. ; Hromas, Robert ; Georgiadis, Millie ; Lee, Suk-Hee. / The DDN catalytic motif is required for Metnase functions in non-homologous end joining (NHEJ) repair and replication restart. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 15. pp. 10930-10938.
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