The DDN catalytic motif is required for Metnase functions in non-homologous end joining (NHEJ) repair and replication restart

Hyun Suk Kim, Qiujia Chen, Sung Kyung Kim, Jac A. Nickoloff, Robert Hromas, Millie M. Georgiadis, Suk Hee Lee

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Background: Metnase, a transposase-containing DNA repair protein, retains DNA cleavage activity with a DDN motif. Results: Substitution with the ancestral transposase DDD/DDE catalytic motif results in a decrease in ssDNA binding and ss-overhang cleavage activities. Conclusion: The DDN motif is required for Metnase DNA repair activities. Significance: Understanding the requirements for catalytic activity provides insights on how Metnase functions as a DNA repair protein.

Original languageEnglish (US)
Pages (from-to)10930-10938
Number of pages9
JournalJournal of Biological Chemistry
Volume289
Issue number15
DOIs
StatePublished - Apr 11 2014

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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