The dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development

J. Q. Feng, H. Huang, Y. Lu, L. Ye, Y. Xie, T. W. Tsutsui, T. Kunieda, T. Castranio, G. Scott, L. B. Bonewald, Y. Mishina

Research output: Contribution to journalArticle

169 Citations (Scopus)

Abstract

Dentin Matrix Protein 1 (Dmp1) was originally identified from dentin. However, its expression and function in vivo are not clear. To clarify these two issues, we have generated mice carrying a truncated Dmp1 gene by using gene targeting to replace exon 6 with a lacZ gene. Northern blot analysis shows the expected 5.8-kb Dmp1-lacZ fusion transcript and loss of the wild-type 2.8-kb Dmp1 transcript, confirmed by a lack of immunostaining for the protein. Using heterozygous animals, we demonstrate that Dmp1 is specific for mineralized tissues. Not previously shown, Dmp1 is also expressed in pulp cells. Dmp1-deficient embryos and newborns display no apparent gross abnormal phenotype, although there are a modest expansion of the hypertrophic chondrocyte zone and a modest increase in the long bone diameter. This suggests that DMP1 is not essential for early mouse skeletal or dental development.

Original languageEnglish (US)
Pages (from-to)776-780
Number of pages5
JournalJournal of dental research
Volume82
Issue number10
DOIs
StatePublished - Oct 2003
Externally publishedYes

Fingerprint

Dentin
Proteins
Lac Operon
Gene Targeting
Chondrocytes
Northern Blotting
Exons
Tooth
Embryonic Structures
Phenotype
Bone and Bones

Keywords

  • Bone
  • Development
  • Dmp1 expression
  • Knockout mice
  • Tooth

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

The dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development. / Feng, J. Q.; Huang, H.; Lu, Y.; Ye, L.; Xie, Y.; Tsutsui, T. W.; Kunieda, T.; Castranio, T.; Scott, G.; Bonewald, L. B.; Mishina, Y.

In: Journal of dental research, Vol. 82, No. 10, 10.2003, p. 776-780.

Research output: Contribution to journalArticle

Feng, JQ, Huang, H, Lu, Y, Ye, L, Xie, Y, Tsutsui, TW, Kunieda, T, Castranio, T, Scott, G, Bonewald, LB & Mishina, Y 2003, 'The dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development', Journal of dental research, vol. 82, no. 10, pp. 776-780. https://doi.org/10.1177/154405910308201003
Feng, J. Q. ; Huang, H. ; Lu, Y. ; Ye, L. ; Xie, Y. ; Tsutsui, T. W. ; Kunieda, T. ; Castranio, T. ; Scott, G. ; Bonewald, L. B. ; Mishina, Y. / The dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development. In: Journal of dental research. 2003 ; Vol. 82, No. 10. pp. 776-780.
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