The development of larger cells that spontaneously escape senescence - A step during the immortalization of a human cancer cell line

Carmen C. Diaconu, Coralia Bleotu, Mihaela Chivu, Irina Alexiu, Daniela Petrusca, Gabriela Anton, Ruxandra Achim, Simona M. Ruta, C. Cernescu

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

There are few information concerning the changes associated with the transition interval when slow growing, primary explanted human cancer cells are displaced by new selected faster growing cells and became an immortal cell line. In a previous paper (J. Cell. Mol. Med., 5: 49-59, 2001) we described the TV cell line derived from a laryngeal tumor which harbors human papillomavirus (HPV) gene sequences throughout more than sixty in vitro passages. In this paper we analyze the modifications observed during the crisis interval when significant amount of cells senesce but occasional cells acquire some mutations that make them immortal. Confocal microscopy analysis revealed the heterogeneity of the cells in terms of their size and nucleus/cell ratio. Proliferation capacity was assessed by flow cytometry analyzing DNA content and expression of transferrin receptor (CD71). We discussed the possibility that HPV genome sequences alleviate a proliferation block during the crisis growth arrest of human larynx carcinoma cell line and the possibility that the cells monitor their size and growth by measuring the levels of some protein whose synthesis is coupled to cell development.

Original languageEnglish (US)
Pages (from-to)93-101
Number of pages9
JournalJournal of Cellular and Molecular Medicine
Volume8
Issue number1
DOIs
StatePublished - Jan 1 2004
Externally publishedYes

Keywords

  • Cancer cell line
  • HPV
  • Immortalization
  • Senescence

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology

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