The direct cytotoxic effects of medicaments used in endodontic regeneration on human dental pulp cells

Nawaf Labban, Ghaeth H. Yassen, L. Windsor, Jeffrey Platt

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Aim: The purpose of this in vitro study was to evaluate the effects of intracanal medicaments commonly used in endodontic regeneration on the survival of human dental pulp cells (DPCs). Methods: DPCs were cultured and exposed to either no medicament treatment or low concentrations (0.3-5 mg ml-1) of calcium hydroxide [Ca(OH)2], triple antibiotic paste (TAP), or double antibiotic paste (DAP) for 3 days. After that, toxicity to the DPCs was determined by lactate dehydrogenase activity assays (LDH) and cell proliferation was measured by colorimetric assays (WST-1). Two-way anova followed by Fisher's protected least significant differences was used for statistical analyses (α = 0.05). Results: The group-by-concentration interactions were significant for the LDH and WST-1 assays (P -1) of Ca(OH)2 and TAP caused significant toxicity to the DPCs compared with the untreated control, while four tested concentrations of DAP (0.5, 1, 2.5, and 5 mg ml-1) caused significant toxicity to the DPCs compared with the untreated control. For the WST-1 assays, the highest concentrations that did not negatively affect the proliferation rate of DAP, TAP and Ca(OH)2 were 0.3, 2, and 2.5 mg ml-1, respectively. Conclusion: The low concentrations of intracanal medicaments tested in this study were not cytotoxic in cultured cells. However, these concentrations are much lower than the concentrations that have been advocated in endodontic regeneration. Furthermore, the negative effects of TAP on DPCs were detected at lower concentrations by using the WST-1 assays than by measuring the LDH release.

Original languageEnglish (US)
Pages (from-to)429-434
Number of pages6
JournalDental Traumatology
Volume30
Issue number6
DOIs
StatePublished - Dec 1 2014

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Dental Pulp
Endodontics
Ointments
Regeneration
Anti-Bacterial Agents
Cultured Cells
Calcium Hydroxide
L-Lactate Dehydrogenase
Cell Proliferation

Keywords

  • Calcium hydroxide
  • Cytotoxicity
  • Double antibiotic paste
  • Endodontic regeneration
  • Triple antibiotic paste

ASJC Scopus subject areas

  • Oral Surgery

Cite this

The direct cytotoxic effects of medicaments used in endodontic regeneration on human dental pulp cells. / Labban, Nawaf; Yassen, Ghaeth H.; Windsor, L.; Platt, Jeffrey.

In: Dental Traumatology, Vol. 30, No. 6, 01.12.2014, p. 429-434.

Research output: Contribution to journalArticle

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