The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells

Joanna L. Parish, Jack Rosa, Xiaoyu Wang, Jill M. Lahti, Stephen J. Doxsey, Elliot Androphy

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

It has recently been suggested that the Saccharomyces cerevisiae protein Chl1p plays a role in cohesion establishment. Here, we show that the human ATP-dependent DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells. Localization studies show that ChlR1 diffusely coats mitotic chromatin in prophase and then translocates from the chromatids to concentrate at the spindle poles during the transition to metaphase. Depletion of ChlR1 protein by RNA interference results in mitotic failure with replicated chromosomes failing to segregate after a pro-metaphase arrest. We show that depletion also results in abnormal sister chromatid cohesion, determined by increased separation of chromatid pairs at the centromere. Furthermore, biochemical studies show that ChlR1 is in complex with cohesin factors Scc1, Smc1 and Smc3. We conclude that human ChlR1 is required for sister chromatid cohesion and, hence, normal mitotic progression. These functions are important to maintain genetic fidelity.

Original languageEnglish (US)
Pages (from-to)4857-4865
Number of pages9
JournalJournal of Cell Science
Volume119
Issue number23
DOIs
StatePublished - Dec 1 2006
Externally publishedYes

Fingerprint

DNA Helicases
Chromatids
Prometaphase
Spindle Poles
Saccharomyces cerevisiae Proteins
Prophase
Centromere
Metaphase
RNA Interference
Chromatin
Chromosomes
Proteins

Keywords

  • Cohesin
  • Cohesion
  • Helicase
  • Mitosis

ASJC Scopus subject areas

  • Cell Biology

Cite this

The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells. / Parish, Joanna L.; Rosa, Jack; Wang, Xiaoyu; Lahti, Jill M.; Doxsey, Stephen J.; Androphy, Elliot.

In: Journal of Cell Science, Vol. 119, No. 23, 01.12.2006, p. 4857-4865.

Research output: Contribution to journalArticle

Parish, Joanna L. ; Rosa, Jack ; Wang, Xiaoyu ; Lahti, Jill M. ; Doxsey, Stephen J. ; Androphy, Elliot. / The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells. In: Journal of Cell Science. 2006 ; Vol. 119, No. 23. pp. 4857-4865.
@article{d94b65f116aa4ae883b077be125e8081,
title = "The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells",
abstract = "It has recently been suggested that the Saccharomyces cerevisiae protein Chl1p plays a role in cohesion establishment. Here, we show that the human ATP-dependent DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells. Localization studies show that ChlR1 diffusely coats mitotic chromatin in prophase and then translocates from the chromatids to concentrate at the spindle poles during the transition to metaphase. Depletion of ChlR1 protein by RNA interference results in mitotic failure with replicated chromosomes failing to segregate after a pro-metaphase arrest. We show that depletion also results in abnormal sister chromatid cohesion, determined by increased separation of chromatid pairs at the centromere. Furthermore, biochemical studies show that ChlR1 is in complex with cohesin factors Scc1, Smc1 and Smc3. We conclude that human ChlR1 is required for sister chromatid cohesion and, hence, normal mitotic progression. These functions are important to maintain genetic fidelity.",
keywords = "Cohesin, Cohesion, Helicase, Mitosis",
author = "Parish, {Joanna L.} and Jack Rosa and Xiaoyu Wang and Lahti, {Jill M.} and Doxsey, {Stephen J.} and Elliot Androphy",
year = "2006",
month = "12",
day = "1",
doi = "10.1242/jcs.03262",
language = "English (US)",
volume = "119",
pages = "4857--4865",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "23",

}

TY - JOUR

T1 - The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells

AU - Parish, Joanna L.

AU - Rosa, Jack

AU - Wang, Xiaoyu

AU - Lahti, Jill M.

AU - Doxsey, Stephen J.

AU - Androphy, Elliot

PY - 2006/12/1

Y1 - 2006/12/1

N2 - It has recently been suggested that the Saccharomyces cerevisiae protein Chl1p plays a role in cohesion establishment. Here, we show that the human ATP-dependent DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells. Localization studies show that ChlR1 diffusely coats mitotic chromatin in prophase and then translocates from the chromatids to concentrate at the spindle poles during the transition to metaphase. Depletion of ChlR1 protein by RNA interference results in mitotic failure with replicated chromosomes failing to segregate after a pro-metaphase arrest. We show that depletion also results in abnormal sister chromatid cohesion, determined by increased separation of chromatid pairs at the centromere. Furthermore, biochemical studies show that ChlR1 is in complex with cohesin factors Scc1, Smc1 and Smc3. We conclude that human ChlR1 is required for sister chromatid cohesion and, hence, normal mitotic progression. These functions are important to maintain genetic fidelity.

AB - It has recently been suggested that the Saccharomyces cerevisiae protein Chl1p plays a role in cohesion establishment. Here, we show that the human ATP-dependent DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells. Localization studies show that ChlR1 diffusely coats mitotic chromatin in prophase and then translocates from the chromatids to concentrate at the spindle poles during the transition to metaphase. Depletion of ChlR1 protein by RNA interference results in mitotic failure with replicated chromosomes failing to segregate after a pro-metaphase arrest. We show that depletion also results in abnormal sister chromatid cohesion, determined by increased separation of chromatid pairs at the centromere. Furthermore, biochemical studies show that ChlR1 is in complex with cohesin factors Scc1, Smc1 and Smc3. We conclude that human ChlR1 is required for sister chromatid cohesion and, hence, normal mitotic progression. These functions are important to maintain genetic fidelity.

KW - Cohesin

KW - Cohesion

KW - Helicase

KW - Mitosis

UR - http://www.scopus.com/inward/record.url?scp=33845657930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845657930&partnerID=8YFLogxK

U2 - 10.1242/jcs.03262

DO - 10.1242/jcs.03262

M3 - Article

VL - 119

SP - 4857

EP - 4865

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 23

ER -