The Drosophila cation channel trpl expressed in insect Sf9 cells is stimulated by agonists of G-protein-coupled receptors

Christian Harteneck, Alexander G. Obukhov, Andrea Zobel, Frank Kalkbrenner, Günter Schultz

Research output: Contribution to journalArticle

75 Scopus citations


Structures and regulations of vertebrate channels responsible for sustained calcium elevations after hormone stimulation are largely unknown. Therefore, the Drosophila photoreceptor channels, trp and trpl, which are assumed to be involved in calcium influx, serve as model system. trpl expressed in Sf9 cells showed spontanous activity. Hormonal stimulations of calcium influx (detected by fura-2) and of an outwardly rectifying current were observed in Sf9 cells coinfected with baculoviruses encoding trpl and various heptahelical receptors for histamine, thrombin, and thromboxane A2, all known to cause phospholipase C-β activation in mammalian cells. Although the identity of the G-proteins and of possible second messengers involved need to be clarified, it is clear that trpl represents a receptor/G-protein regulated cation channel.

Original languageEnglish (US)
Pages (from-to)297-300
Number of pages4
JournalFEBS Letters
Issue number3
StatePublished - Jan 30 1995



  • Baculovirus/Sf9 system
  • Drosophila ion channel
  • G-protein
  • Hormonal effects
  • Non-selective cation channel
  • trpl

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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