The Drosophila cation channel trpl expressed in insect Sf9 cells is stimulated by agonists of G-protein-coupled receptors

Christian Harteneck, Alexander Obukhov, Andrea Zobel, Frank Kalkbrenner, Günter Schultz

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Structures and regulations of vertebrate channels responsible for sustained calcium elevations after hormone stimulation are largely unknown. Therefore, the Drosophila photoreceptor channels, trp and trpl, which are assumed to be involved in calcium influx, serve as model system. trpl expressed in Sf9 cells showed spontanous activity. Hormonal stimulations of calcium influx (detected by fura-2) and of an outwardly rectifying current were observed in Sf9 cells coinfected with baculoviruses encoding trpl and various heptahelical receptors for histamine, thrombin, and thromboxane A2, all known to cause phospholipase C-β activation in mammalian cells. Although the identity of the G-proteins and of possible second messengers involved need to be clarified, it is clear that trpl represents a receptor/G-protein regulated cation channel.

Original languageEnglish (US)
Pages (from-to)297-300
Number of pages4
JournalFEBS Letters
Volume358
Issue number3
DOIs
StatePublished - Jan 30 1995
Externally publishedYes

Fingerprint

Sf9 Cells
G-Protein-Coupled Receptors
Drosophila
Insects
Cations
Calcium
GTP-Binding Proteins
Thrombin Receptors
Histamine Receptors
Thromboxane A2
Fura-2
Baculoviridae
Type C Phospholipases
Second Messenger Systems
Thrombin
Vertebrates
Chemical activation
Cells
Hormones

Keywords

  • Baculovirus/Sf9 system
  • Drosophila ion channel
  • G-protein
  • Hormonal effects
  • Non-selective cation channel
  • trpl

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

The Drosophila cation channel trpl expressed in insect Sf9 cells is stimulated by agonists of G-protein-coupled receptors. / Harteneck, Christian; Obukhov, Alexander; Zobel, Andrea; Kalkbrenner, Frank; Schultz, Günter.

In: FEBS Letters, Vol. 358, No. 3, 30.01.1995, p. 297-300.

Research output: Contribution to journalArticle

Harteneck, Christian ; Obukhov, Alexander ; Zobel, Andrea ; Kalkbrenner, Frank ; Schultz, Günter. / The Drosophila cation channel trpl expressed in insect Sf9 cells is stimulated by agonists of G-protein-coupled receptors. In: FEBS Letters. 1995 ; Vol. 358, No. 3. pp. 297-300.
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