The E3 Ligase MuRF1 Degrades Myosin Heavy Chain Protein in Dexamethasone-Treated Skeletal Muscle

Brian A. Clarke, Doreen Drujan, Monte S. Willis, Leon O. Murphy, Richard A. Corpina, Elena Burova, Sergey V. Rakhilin, Trevor N. Stitt, Cam Patterson, Esther Latres, David J. Glass

Research output: Contribution to journalArticle

392 Scopus citations

Abstract

Skeletal muscle atrophy occurs as a side effect of treatment with synthetic glucocorticoids such as dexamethasone (DEX) and is a hallmark of cachectic syndromes associated with increased cortisol levels. The E3 ubiquitin ligase MuRF1 (muscle RING finger protein 1) is transcriptionally upregulated by DEX treatment. Differentiated myotubes treated with DEX undergo depletion of myosin heavy chain protein (MYH), which physically associates with MuRF1. This loss of MYH can be blocked by inhibition of MuRF1 expression. When wild-type and MuRF1-/- mice are treated with DEX, the MuRF1-/- animals exhibit a relative sparing of MYH. In vitro, MuRF1 is shown to function as an E3 ubiquitin ligase for MYH. These data identify the mechanism by which MYH is depleted under atrophy conditions and demonstrate that inhibition of a single E3 ligase, MuRF1, is sufficient to maintain this important sarcomeric protein.

Original languageEnglish (US)
Pages (from-to)376-385
Number of pages10
JournalCell Metabolism
Volume6
Issue number5
DOIs
StatePublished - Nov 7 2007
Externally publishedYes

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Keywords

  • HUMDISEASE

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

Cite this

Clarke, B. A., Drujan, D., Willis, M. S., Murphy, L. O., Corpina, R. A., Burova, E., Rakhilin, S. V., Stitt, T. N., Patterson, C., Latres, E., & Glass, D. J. (2007). The E3 Ligase MuRF1 Degrades Myosin Heavy Chain Protein in Dexamethasone-Treated Skeletal Muscle. Cell Metabolism, 6(5), 376-385. https://doi.org/10.1016/j.cmet.2007.09.009