The E5 protein of human papillomavirus type 16 perturbs MHC class II antigen maturation in human foreskin keratinocytes treated with interferon-γ

Benyue Zhang, Ping Li, Exing Wang, Zacharie Brahmi, Kenneth Dunn, Janice Blum, Ann Roman

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Major histocompatibility complex (MHC) class II antigens are expressed on human foreskin keratinocytes (HFKs) following exposure to interferon gamma. The expression of MHC class II proteins on the cell surface may allow keratinocytes to function as antigen-presenting cells and induce a subsequent immune response to virus infection. Invariant chain (Ii) is a chaperone protein which plays an important role in the maturation of MHC class II molecules. The sequential degradation of Ii within acidic endocytic compartments is a key process required for the successful loading of antigenic peptide onto MHC class II molecules. Since human papillomavirus (HPV) 16 E5 can inhibit the acidification of late endosomes in HFKs, the E5 protein may be able to affect proper peptide loading onto the MHC class II molecule. To test this hypothesis, HFKs were infected with either control virus or a recombinant virus expressing HPV16 E5 and the infected cells were subsequently treated with interferon-γ. ELISAs revealed a decrease of MHC class II expression on the surface of E5-expressing cells compared with control virus-infected cells after interferon treatment. Western blot analysis showed that, in cells treated with interferon gamma, E5 could prevent the breakdown of Ii and block the formation of peptide-loaded, SDS-stable mature MHC class II dimers, correlating with diminished surface MHC class II expression. These data suggest that HPV16 E5 may be able to decrease immune recognition of infected keratinocytes via disruption of MHC class II protein function.

Original languageEnglish
Pages (from-to)100-108
Number of pages9
JournalVirology
Volume310
Issue number1
DOIs
StatePublished - May 25 2003

Fingerprint

Foreskin
Histocompatibility Antigens Class II
Major Histocompatibility Complex
Keratinocytes
Interferons
Viruses
Peptides
Interferon-gamma
oncogene protein E5, Human papillomavirus type 16
Proteins
Human papillomavirus 16
Endosomes
Antigen-Presenting Cells
Virus Diseases
Membrane Proteins
Western Blotting
Enzyme-Linked Immunosorbent Assay

Keywords

  • E5 protein
  • Human papillomavirus
  • Keratinocytes
  • MHC class II maturation

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

The E5 protein of human papillomavirus type 16 perturbs MHC class II antigen maturation in human foreskin keratinocytes treated with interferon-γ. / Zhang, Benyue; Li, Ping; Wang, Exing; Brahmi, Zacharie; Dunn, Kenneth; Blum, Janice; Roman, Ann.

In: Virology, Vol. 310, No. 1, 25.05.2003, p. 100-108.

Research output: Contribution to journalArticle

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