The Ebola virus matrix protein deeply penetrates the plasma membrane

An important step in viral egress

Smita P. Soni, Emmanuel Adu-Gyamfi, Sylvia S. Yong, Clara S. Jee, Robert Stahelin

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Ebola virus, from the Filoviridae family has a high fatality rate in humans and nonhuman primates and to date, to the best of our knowledge, has no FDA approved vaccines or therapeutics. Viral protein 40 (VP40) is the major Ebola virus matrix protein that regulates assembly and egress of infectious Ebola virus particles. It is well established that VP40 assembles on the inner leaflet of the plasma membrane; however, the mechanistic details of VP40 membrane binding that are important for viral release remain to be elucidated. In this study, we used fluorescence quenching of a tryptophan on the membrane-binding interface with brominated lipids along with mutagenesis of VP40 to understand the depth of membrane penetration into lipid bilayers. Experimental results indicate that VP40 penetrates 8.1 Å into the hydrocarbon core of the plasma membrane bilayer. VP40 also induces substantial changes to membrane curvature as it tubulates liposomes and induces vesiculation into giant unilamellar vesicles, effects that are abrogated by hydrophobic mutations. This is a critical step in viral egress as cellular assays demonstrate that hydrophobic residues that penetrate deeply into the plasma membrane are essential for plasma membrane localization and virus-like particle formation and release from cells.

Original languageEnglish
Pages (from-to)1940-1949
Number of pages10
JournalBiophysical Journal
Volume104
Issue number9
DOIs
StatePublished - May 7 2013

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Ebolavirus
Viral Proteins
Cell Membrane
Proteins
Virion
Filoviridae
Membranes
Viral Matrix Proteins
Unilamellar Liposomes
Lipid Bilayers
Hydrocarbons
Liposomes
Tryptophan
Mutagenesis
Primates
Vaccines
Fluorescence
Lipids
Mutation

ASJC Scopus subject areas

  • Biophysics

Cite this

The Ebola virus matrix protein deeply penetrates the plasma membrane : An important step in viral egress. / Soni, Smita P.; Adu-Gyamfi, Emmanuel; Yong, Sylvia S.; Jee, Clara S.; Stahelin, Robert.

In: Biophysical Journal, Vol. 104, No. 9, 07.05.2013, p. 1940-1949.

Research output: Contribution to journalArticle

Soni, Smita P. ; Adu-Gyamfi, Emmanuel ; Yong, Sylvia S. ; Jee, Clara S. ; Stahelin, Robert. / The Ebola virus matrix protein deeply penetrates the plasma membrane : An important step in viral egress. In: Biophysical Journal. 2013 ; Vol. 104, No. 9. pp. 1940-1949.
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