The ebola virus matrix protein VP40 selectively induces vesiculation from phosphatidylserine-enriched membranes

Smita P. Soni, Robert Stahelin

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Ebola virus is from the Filoviridae family of viruses and is one of the most virulent pathogens known with ∼60% clinical fatality. The Ebola virus negative sense RNA genome encodes seven proteins including viral matrix protein 40 (VP40), which is the most abundant protein found in the virions. Within infected cells VP40 localizes at the inner leaflet of the plasma membrane (PM), binds lipids, and regulates formation of new virus particles. Expression of VP40 in mammalian cells is sufficient to form virus-like particles that are nearly indistinguishable from the authentic virions. However, how VP40 interacts with the PM and forms virus-like particles is for the most part unknown. To investigate VP40 lipid specificity in a model of viral egress we employed giant unilamellar vesicles with different lipid compositions. The results demonstrate VP40 selectively induces vesiculation from membranes containing phosphatidylserine (PS) at concentrations of PS that are representative of the PM inner leaflet content. The formation of intraluminal vesicles was not significantly detected in the presence of other important PM lipids including cholesterol and polyvalent phosphoinositides, further demonstrating PS selectivity. Taken together, these studies suggest that PM phosphatidylserine may be an important component of Ebola virus budding and that VP40 may be able to mediate PM scission.

Original languageEnglish
Pages (from-to)33590-33597
Number of pages8
JournalJournal of Biological Chemistry
Volume289
Issue number48
DOIs
StatePublished - Nov 28 2014

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Viral Matrix Proteins
Ebolavirus
Phosphatidylserines
Viruses
Cell membranes
Virion
Proteins
Membranes
Cell Membrane
Membrane Lipids
Filoviridae
Virus Release
Lipids
Unilamellar Liposomes
Pathogens
Phosphatidylinositols
Genes
Cholesterol
Cells
Genome

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

The ebola virus matrix protein VP40 selectively induces vesiculation from phosphatidylserine-enriched membranes. / Soni, Smita P.; Stahelin, Robert.

In: Journal of Biological Chemistry, Vol. 289, No. 48, 28.11.2014, p. 33590-33597.

Research output: Contribution to journalArticle

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