Olfactory epithelium (OE) is a unique tissue since its components, including olfactory receptor neurons, turnover throughout life. Previous studies in our lab have demonstrated that: in situ chemical trauma to the adult mouse OE, several days prior to removal, facilitates the development of heterogeneous cultures. Further characterization reveals four distinct cell types: bipolar cells which have neuronal markers; fusiform cells which are relatively small and undifferentiated; clusters of nest cells with the morphological characteristics of epithelia that are keratin positive and giant cells which remain as solitary flat cells that appear unique to the adult trauma paradigm. This study examined the effect of basic Fibroblast Growth Factor (bFOF) on OE cultures. bFGF was incorporated into the medium at 3 days in vitro over a range of concentrations (10-50 ng/ml). Four and eight days following exposure, the number of each cell type was determined, as was the mean neunte length of bipolar cells. bFGF stimulated the neunte development of bipolar cells, the neunte length increased at all concentrations, evaluated (P < 0.001), however, the change was not dose-dependent. bFGF treatment also induced an increase in bipolar cells (P < 0.001) and decrease in giant cells (P < 0.001). Following the exposure to bFGF, a subpopulation of giant cells extended long processes and gradually transformed into bipolar cells. Our data suggests that bFGF promotes the differentiation of bipolar cell population and increases their neuritogenic activity. This response was not dose-dependent, therefore, bFGF might act as a trigger to turn on neuritogenesis. Furthermore, bFGF appeared to enhance the transformation of fusiform cells but did not affect the nest cells. This study demonstrates the utility of olfactory epithelial model for the study of trophic interaction.
|Original language||English (US)|
|State||Published - Mar 20 1998|
ASJC Scopus subject areas
- Molecular Biology