The effect of dichloromethylene bisphosphonate on human gingival crevicular neutrophil myeloperoxidase activity

M. J. Kowolik, P. M. Hyvonen

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Neutrophil myeloperoxidase is an important component of the oxygen-dependent microbicidal system and is enzymatically activated in crevicular cells. The purpose of this study was to investigate the effect of dichloromethylene bisphosphonate, a drug used to modify bone resorption, on extracted neutrophil myeloperoxidase activity directly, and indirectly, by means of a luminol-dependent chemiluminescence assay. Enzyme activity was increased, up to a drug concentration of 1 × 102 μg/ml: 1 × 105 neutrophils. Inhibition occurred only at high concentrations, supporting the evidence for low toxicity. This cellular model warrants further investigation with respect both to possible modulation of inflammatory mechanisms relevant to the pathogenesis of periodontitis and other diseases, and to a better understanding of the pharmacological activity of bisphosphonates.

Original languageEnglish (US)
Pages (from-to)S201-S203
JournalArchives of Oral Biology
Volume35
Issue numberSUPPL.
DOIs
StatePublished - 1990
Externally publishedYes

Keywords

  • dichloromethylene bisphosphate
  • myeloperoxidase
  • neutrophil
  • periodontal disease

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

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