The effect of dichloromethylene bisphosphonate on human gingival crevicular neutrophil myeloperoxidase activity

Michael Kowolik, P. M. Hyvonen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Neutrophil myeloperoxidase is an important component of the oxygen-dependent microbicidal system and is enzymatically activated in crevicular cells. The purpose of this study was to investigate the effect of dichloromethylene bisphosphonate, a drug used to modify bone resorption, on extracted neutrophil myeloperoxidase activity directly, and indirectly, by means of a luminol-dependent chemiluminescence assay. Enzyme activity was increased, up to a drug concentration of 1 × 102 μg/ml: 1 × 105 neutrophils. Inhibition occurred only at high concentrations, supporting the evidence for low toxicity. This cellular model warrants further investigation with respect both to possible modulation of inflammatory mechanisms relevant to the pathogenesis of periodontitis and other diseases, and to a better understanding of the pharmacological activity of bisphosphonates.

Original languageEnglish (US)
JournalArchives of Oral Biology
Volume35
Issue numberSUPPL.
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Diphosphonates
Peroxidase
Neutrophils
Luminol
Periodontitis
Bone Resorption
Luminescence
Pharmaceutical Preparations
Pharmacology
Oxygen
Enzymes

Keywords

  • dichloromethylene bisphosphate
  • myeloperoxidase
  • neutrophil
  • periodontal disease

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

The effect of dichloromethylene bisphosphonate on human gingival crevicular neutrophil myeloperoxidase activity. / Kowolik, Michael; Hyvonen, P. M.

In: Archives of Oral Biology, Vol. 35, No. SUPPL., 1990.

Research output: Contribution to journalArticle

@article{b8a96fa68ca8474e95495545e3ea4e95,
title = "The effect of dichloromethylene bisphosphonate on human gingival crevicular neutrophil myeloperoxidase activity",
abstract = "Neutrophil myeloperoxidase is an important component of the oxygen-dependent microbicidal system and is enzymatically activated in crevicular cells. The purpose of this study was to investigate the effect of dichloromethylene bisphosphonate, a drug used to modify bone resorption, on extracted neutrophil myeloperoxidase activity directly, and indirectly, by means of a luminol-dependent chemiluminescence assay. Enzyme activity was increased, up to a drug concentration of 1 × 102 μg/ml: 1 × 105 neutrophils. Inhibition occurred only at high concentrations, supporting the evidence for low toxicity. This cellular model warrants further investigation with respect both to possible modulation of inflammatory mechanisms relevant to the pathogenesis of periodontitis and other diseases, and to a better understanding of the pharmacological activity of bisphosphonates.",
keywords = "dichloromethylene bisphosphate, myeloperoxidase, neutrophil, periodontal disease",
author = "Michael Kowolik and Hyvonen, {P. M.}",
year = "1990",
doi = "10.1016/0003-9969(90)90158-7",
language = "English (US)",
volume = "35",
journal = "Archives of Oral Biology",
issn = "0003-9969",
publisher = "Elsevier Limited",
number = "SUPPL.",

}

TY - JOUR

T1 - The effect of dichloromethylene bisphosphonate on human gingival crevicular neutrophil myeloperoxidase activity

AU - Kowolik, Michael

AU - Hyvonen, P. M.

PY - 1990

Y1 - 1990

N2 - Neutrophil myeloperoxidase is an important component of the oxygen-dependent microbicidal system and is enzymatically activated in crevicular cells. The purpose of this study was to investigate the effect of dichloromethylene bisphosphonate, a drug used to modify bone resorption, on extracted neutrophil myeloperoxidase activity directly, and indirectly, by means of a luminol-dependent chemiluminescence assay. Enzyme activity was increased, up to a drug concentration of 1 × 102 μg/ml: 1 × 105 neutrophils. Inhibition occurred only at high concentrations, supporting the evidence for low toxicity. This cellular model warrants further investigation with respect both to possible modulation of inflammatory mechanisms relevant to the pathogenesis of periodontitis and other diseases, and to a better understanding of the pharmacological activity of bisphosphonates.

AB - Neutrophil myeloperoxidase is an important component of the oxygen-dependent microbicidal system and is enzymatically activated in crevicular cells. The purpose of this study was to investigate the effect of dichloromethylene bisphosphonate, a drug used to modify bone resorption, on extracted neutrophil myeloperoxidase activity directly, and indirectly, by means of a luminol-dependent chemiluminescence assay. Enzyme activity was increased, up to a drug concentration of 1 × 102 μg/ml: 1 × 105 neutrophils. Inhibition occurred only at high concentrations, supporting the evidence for low toxicity. This cellular model warrants further investigation with respect both to possible modulation of inflammatory mechanisms relevant to the pathogenesis of periodontitis and other diseases, and to a better understanding of the pharmacological activity of bisphosphonates.

KW - dichloromethylene bisphosphate

KW - myeloperoxidase

KW - neutrophil

KW - periodontal disease

UR - http://www.scopus.com/inward/record.url?scp=0025618846&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025618846&partnerID=8YFLogxK

U2 - 10.1016/0003-9969(90)90158-7

DO - 10.1016/0003-9969(90)90158-7

M3 - Article

C2 - 1965118

AN - SCOPUS:0025618846

VL - 35

JO - Archives of Oral Biology

JF - Archives of Oral Biology

SN - 0003-9969

IS - SUPPL.

ER -