The effect of dynamic compression on the response of articular cartilage to insulin-like growth factor-I

Lawrence J. Bonassar, Alan J. Grodzinsky, Eliot H. Frank, Salomon G. Davila, Nirav R. Bhaktav, Stephen Trippel

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

Articular cartilage is routinely subjected to mechanical forces and to cell-regulatory molecules. Previous studies have shown that mechanical stimuli can influence articular chondrocyte metabolic activity, and biochemical studies have shown that growth factors and cytokines control many of the same cell functions. Little is known, however, of the relationships or interplay, if any, between these two key components of the articular environment. This study investigated the comparative and interactive effects of low amplitude, sinusoidal, dynamic compression and insulin-like growth factor-I (IGF-I), a polypeptide in synovial fluid that is anabolic for cartilage. In bovine patellofemoral cartilage explants, IGF-I increased protein and proteoglycan synthesis 90% and 120%, respectively while dynamic compression increased protein and proteoglycan synthesis 40% and 90%, respectively. Stimulation by IGF-I was significantly greater than by dynamic compression for both protein and proteoglycan synthesis. When applied together, the two stimuli enhanced protein and proteoglycan synthesis by 180% and 290%, respectively, a degree than that achieved by either stimulus alone. IGF-I augmented protein synthesis with a time constant of 12.2 h. Dynamic compression increased protein synthesis with a time constant of 2.9 h, a rate significantly faster than that of IGF-I, suggesting that these signals act via distinct cell activation pathways. When used together, dynamic compression and IGF-I acted with time constant of 5.6 h. Thus, dynamic compression accelerated the biosynthetic response to IGF-I and increased transport of IGF-I into the articular cartilage matrix, suggesting that, in addition to independently stimulating articular chondrocytes, cyclic compression may improve the access of soluble growth factors to these relatively isolated cells.

Original languageEnglish (US)
Pages (from-to)11-17
Number of pages7
JournalJournal of Orthopaedic Research
Volume19
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Articular Cartilage
Insulin-Like Growth Factor I
Proteoglycans
Proteins
Joints
Chondrocytes
Cartilage
Intercellular Signaling Peptides and Proteins
Synovial Fluid
Cytokines
Peptides

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Bonassar, L. J., Grodzinsky, A. J., Frank, E. H., Davila, S. G., Bhaktav, N. R., & Trippel, S. (2001). The effect of dynamic compression on the response of articular cartilage to insulin-like growth factor-I. Journal of Orthopaedic Research, 19(1), 11-17. https://doi.org/10.1016/S0736-0266(00)00004-8

The effect of dynamic compression on the response of articular cartilage to insulin-like growth factor-I. / Bonassar, Lawrence J.; Grodzinsky, Alan J.; Frank, Eliot H.; Davila, Salomon G.; Bhaktav, Nirav R.; Trippel, Stephen.

In: Journal of Orthopaedic Research, Vol. 19, No. 1, 2001, p. 11-17.

Research output: Contribution to journalArticle

Bonassar, Lawrence J. ; Grodzinsky, Alan J. ; Frank, Eliot H. ; Davila, Salomon G. ; Bhaktav, Nirav R. ; Trippel, Stephen. / The effect of dynamic compression on the response of articular cartilage to insulin-like growth factor-I. In: Journal of Orthopaedic Research. 2001 ; Vol. 19, No. 1. pp. 11-17.
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