The effect of low oxygen with and without steady-state hydrogen peroxide on cytokine gene and protein expression of monocyte-derived macrophages

Henry Owegi, Hongtao Li, Mark Bouwens, Stephane Egot-Lemaire, Sebastian Mueller, Roy W. Geib, Gabi N. Waite

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An early event during inflammation and infection is the migration of monocytes into tissues where they differentiate into macrophages. Such monocyte-derived macrophages face an unfavorable environment characterized by extremely low oxygen tension and accumulation of reactive oxygen species such as hydrogen peroxide. Previous experiments showed that a macrophage cell line cultured under these conditions responded to inflammatory stimulants with an increased respiratory burst compared to cells kept at regular ambient oxygen conditions. The objective of the present study was to test if this effect was accompanied by an increased c ytokineproduction. Using cytokine protein arrays and real time gene expression analysis, we indeed found that low oxygen exposure increased expression of characteristic macrophage inflammatory cytokines such as IL-1, IL-6, and TNF-alpha. Interestingly, cells exposed to low oxygen and steady-state hydrogen peroxide levels did not show this increase, indicating different cell mechanisms might be involved in increasing macrophage hydrogen peroxide and cytokine release.

Original languageEnglish
Pages (from-to)58-63
Number of pages6
JournalBiomedical Sciences Instrumentation
Volume47
StatePublished - 2011

Fingerprint

Hydrogen Peroxide
Macrophages
Cytokines
Oxygen
Gene Expression
Proteins
Protein Array Analysis
Respiratory Burst
Interleukin-1
Monocytes
Interleukin-6
Reactive Oxygen Species
Tumor Necrosis Factor-alpha
Inflammation
Cell Line
Infection

Keywords

  • Chemokines
  • Cytokines
  • Ho
  • HIF-1α
  • Hypoxia
  • Inflammation
  • O
  • Respiratory burst
  • THP-1
  • Transcription factors

ASJC Scopus subject areas

  • Medical Laboratory Technology
  • Biophysics

Cite this

The effect of low oxygen with and without steady-state hydrogen peroxide on cytokine gene and protein expression of monocyte-derived macrophages. / Owegi, Henry; Li, Hongtao; Bouwens, Mark; Egot-Lemaire, Stephane; Mueller, Sebastian; Geib, Roy W.; Waite, Gabi N.

In: Biomedical Sciences Instrumentation, Vol. 47, 2011, p. 58-63.

Research output: Contribution to journalArticle

Owegi, Henry ; Li, Hongtao ; Bouwens, Mark ; Egot-Lemaire, Stephane ; Mueller, Sebastian ; Geib, Roy W. ; Waite, Gabi N. / The effect of low oxygen with and without steady-state hydrogen peroxide on cytokine gene and protein expression of monocyte-derived macrophages. In: Biomedical Sciences Instrumentation. 2011 ; Vol. 47. pp. 58-63.
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AU - Waite, Gabi N.

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