The effect of postirradiation holding at 22°C on the repair of sublethal, potentially lethal and potentially neoplastic transforming damage in gamma- irradiated HeLa x skin fibroblast human hybrid cells

J. L. Redpath, R. J. Antoniono, Marc Mendonca, C. Sun

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3 Citations (Scopus)

Abstract

The effect of postirradiation holding at 22°C on cell growth, progression of cells through the cell cycle, and the repair of sublethal, potentially lethal and potentially neoplastic transforming damage in γ-irradiated HeLa x skin fibroblast human hybrid cells has been examined. Cell growth and cell cycle progression were essentially stopped at this reduced temperature. Cell survival was dramatically reduced by holding confluent cultures for 6 h at 22°C, as opposed to 37°C, after 7.5 Gy γ radiation delivered at a rate of 2 Gy/min. Return of the cells to 37°C for 6 h after holding at 22°C did not result in increased survival. A similar effect was obtained when the cells were held at 22°C between split-dose irradiation of log-phase cultures where no increase in survival was observed over a split-dose interval of 4 h. In this case a partial increase in survival was observed upon returning the cells to 37°C for 3 h after holding at 22°C for the first 3 h of the split- dose interval. Neoplastic transformation frequency was not enhanced by holding confluent cultures for 6 h at 22°C after 7.5 Gy γ radiation. This is consistent with previous observations that misrepair of potentially neoplastic transforming damage already occurs at 37°C. The overall results are interpreted in terms of the reduced temperature favoring misrepair, rather than inhibition of repair, of sublethal, potentially lethal and potentially transforming radiation damage.

Original languageEnglish (US)
Pages (from-to)323-329
Number of pages7
JournalRadiation Research
Volume137
Issue number3
StatePublished - 1994
Externally publishedYes

Fingerprint

Hybrid Cells
fibroblasts
skin (animal)
Fibroblasts
damage
Skin
cells
Radiation Dosage
cell growth
cell cycle
progressions
dosage
Survival
Cell Cycle
intervals
cycles
Temperature
cell viability
radiation
Growth

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

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title = "The effect of postirradiation holding at 22°C on the repair of sublethal, potentially lethal and potentially neoplastic transforming damage in gamma- irradiated HeLa x skin fibroblast human hybrid cells",
abstract = "The effect of postirradiation holding at 22°C on cell growth, progression of cells through the cell cycle, and the repair of sublethal, potentially lethal and potentially neoplastic transforming damage in γ-irradiated HeLa x skin fibroblast human hybrid cells has been examined. Cell growth and cell cycle progression were essentially stopped at this reduced temperature. Cell survival was dramatically reduced by holding confluent cultures for 6 h at 22°C, as opposed to 37°C, after 7.5 Gy γ radiation delivered at a rate of 2 Gy/min. Return of the cells to 37°C for 6 h after holding at 22°C did not result in increased survival. A similar effect was obtained when the cells were held at 22°C between split-dose irradiation of log-phase cultures where no increase in survival was observed over a split-dose interval of 4 h. In this case a partial increase in survival was observed upon returning the cells to 37°C for 3 h after holding at 22°C for the first 3 h of the split- dose interval. Neoplastic transformation frequency was not enhanced by holding confluent cultures for 6 h at 22°C after 7.5 Gy γ radiation. This is consistent with previous observations that misrepair of potentially neoplastic transforming damage already occurs at 37°C. The overall results are interpreted in terms of the reduced temperature favoring misrepair, rather than inhibition of repair, of sublethal, potentially lethal and potentially transforming radiation damage.",
author = "Redpath, {J. L.} and Antoniono, {R. J.} and Marc Mendonca and C. Sun",
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T1 - The effect of postirradiation holding at 22°C on the repair of sublethal, potentially lethal and potentially neoplastic transforming damage in gamma- irradiated HeLa x skin fibroblast human hybrid cells

AU - Redpath, J. L.

AU - Antoniono, R. J.

AU - Mendonca, Marc

AU - Sun, C.

PY - 1994

Y1 - 1994

N2 - The effect of postirradiation holding at 22°C on cell growth, progression of cells through the cell cycle, and the repair of sublethal, potentially lethal and potentially neoplastic transforming damage in γ-irradiated HeLa x skin fibroblast human hybrid cells has been examined. Cell growth and cell cycle progression were essentially stopped at this reduced temperature. Cell survival was dramatically reduced by holding confluent cultures for 6 h at 22°C, as opposed to 37°C, after 7.5 Gy γ radiation delivered at a rate of 2 Gy/min. Return of the cells to 37°C for 6 h after holding at 22°C did not result in increased survival. A similar effect was obtained when the cells were held at 22°C between split-dose irradiation of log-phase cultures where no increase in survival was observed over a split-dose interval of 4 h. In this case a partial increase in survival was observed upon returning the cells to 37°C for 3 h after holding at 22°C for the first 3 h of the split- dose interval. Neoplastic transformation frequency was not enhanced by holding confluent cultures for 6 h at 22°C after 7.5 Gy γ radiation. This is consistent with previous observations that misrepair of potentially neoplastic transforming damage already occurs at 37°C. The overall results are interpreted in terms of the reduced temperature favoring misrepair, rather than inhibition of repair, of sublethal, potentially lethal and potentially transforming radiation damage.

AB - The effect of postirradiation holding at 22°C on cell growth, progression of cells through the cell cycle, and the repair of sublethal, potentially lethal and potentially neoplastic transforming damage in γ-irradiated HeLa x skin fibroblast human hybrid cells has been examined. Cell growth and cell cycle progression were essentially stopped at this reduced temperature. Cell survival was dramatically reduced by holding confluent cultures for 6 h at 22°C, as opposed to 37°C, after 7.5 Gy γ radiation delivered at a rate of 2 Gy/min. Return of the cells to 37°C for 6 h after holding at 22°C did not result in increased survival. A similar effect was obtained when the cells were held at 22°C between split-dose irradiation of log-phase cultures where no increase in survival was observed over a split-dose interval of 4 h. In this case a partial increase in survival was observed upon returning the cells to 37°C for 3 h after holding at 22°C for the first 3 h of the split- dose interval. Neoplastic transformation frequency was not enhanced by holding confluent cultures for 6 h at 22°C after 7.5 Gy γ radiation. This is consistent with previous observations that misrepair of potentially neoplastic transforming damage already occurs at 37°C. The overall results are interpreted in terms of the reduced temperature favoring misrepair, rather than inhibition of repair, of sublethal, potentially lethal and potentially transforming radiation damage.

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