The effect of scatter laser photocoagulation on intravitreal levels of growth factors in the miniature pig

Ming Xiao, Asud Khaliq, Patrick Moriarty, David McLeod, John Cranley, Mike Boulton

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose. To characterise changes in intravitreal growth factor profiles following retinal photocoagulation in the miniature pig. Methods. Miniature pig eyes underwent scatter photocoagulation by either diode infrared or emerald green laser. Animals were sacrificed at various times (up to 42 days) post-laser. The eyes were then removed and vitreous samples analysed for basic fibroblast growth factor, insulin-like growth factor-I and epidermal growth factor by radioimmunoassay, transforming growth factor-beta2 by ELISA and insulin-like growth factor binding proteins using Western ligand blotting. Results. Vitreous transforming growth factor-beta2 levels were decreased at 1 h post diode laser and at 4 and 7 days post emerald laser but returned to normal by 21 and 42 days respectively. Vitreous insulin-like growth factor-I levels increased at 4 and 7 days post diode and emerald laser respectively but returned to normal by 21 days. Insulin-like growth factor Western ligand blotting demonstrated that a 34 kDa insulin-like growth factor binding protein was predominant in the pig vitreous; the levels of this binding protein followed an identical trend to those observed for insulin-like growth factor-I. No changes in vitreous levels of either basic fibroblast growth factor or epidermal growth factor were observed following laser treatment. Conclusions. Our results demonstrate a significant shift in the balance of intravitreal growth factors following retinal laser photocoagulation. Such changes may be pertinent to the regression of preretinal new vessels after laser photocoagulation.

Original languageEnglish (US)
Pages (from-to)923-931
Number of pages9
JournalCurrent Eye Research
Volume15
Issue number9
StatePublished - 1996
Externally publishedYes

Fingerprint

Light Coagulation
Intercellular Signaling Peptides and Proteins
Lasers
Swine
Transforming Growth Factor beta2
Insulin-Like Growth Factor I
Insulin-Like Growth Factor Binding Proteins
Semiconductor Lasers
Fibroblast Growth Factor 2
Epidermal Growth Factor
Western Blotting
Ligands
Somatomedins
Radioimmunoassay
Carrier Proteins
Enzyme-Linked Immunosorbent Assay

Keywords

  • Basic fibroblast growth factor
  • Epidermal growth factor
  • Growth factors
  • Insulin-like growth factor
  • Insulin-like growth factor (IGF)-binding proteins
  • Pig
  • Retinal photocoagulation
  • Transforming growth factor-beta 2
  • Vitreous

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Xiao, M., Khaliq, A., Moriarty, P., McLeod, D., Cranley, J., & Boulton, M. (1996). The effect of scatter laser photocoagulation on intravitreal levels of growth factors in the miniature pig. Current Eye Research, 15(9), 923-931.

The effect of scatter laser photocoagulation on intravitreal levels of growth factors in the miniature pig. / Xiao, Ming; Khaliq, Asud; Moriarty, Patrick; McLeod, David; Cranley, John; Boulton, Mike.

In: Current Eye Research, Vol. 15, No. 9, 1996, p. 923-931.

Research output: Contribution to journalArticle

Xiao, M, Khaliq, A, Moriarty, P, McLeod, D, Cranley, J & Boulton, M 1996, 'The effect of scatter laser photocoagulation on intravitreal levels of growth factors in the miniature pig', Current Eye Research, vol. 15, no. 9, pp. 923-931.
Xiao M, Khaliq A, Moriarty P, McLeod D, Cranley J, Boulton M. The effect of scatter laser photocoagulation on intravitreal levels of growth factors in the miniature pig. Current Eye Research. 1996;15(9):923-931.
Xiao, Ming ; Khaliq, Asud ; Moriarty, Patrick ; McLeod, David ; Cranley, John ; Boulton, Mike. / The effect of scatter laser photocoagulation on intravitreal levels of growth factors in the miniature pig. In: Current Eye Research. 1996 ; Vol. 15, No. 9. pp. 923-931.
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AU - Cranley, John

AU - Boulton, Mike

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N2 - Purpose. To characterise changes in intravitreal growth factor profiles following retinal photocoagulation in the miniature pig. Methods. Miniature pig eyes underwent scatter photocoagulation by either diode infrared or emerald green laser. Animals were sacrificed at various times (up to 42 days) post-laser. The eyes were then removed and vitreous samples analysed for basic fibroblast growth factor, insulin-like growth factor-I and epidermal growth factor by radioimmunoassay, transforming growth factor-beta2 by ELISA and insulin-like growth factor binding proteins using Western ligand blotting. Results. Vitreous transforming growth factor-beta2 levels were decreased at 1 h post diode laser and at 4 and 7 days post emerald laser but returned to normal by 21 and 42 days respectively. Vitreous insulin-like growth factor-I levels increased at 4 and 7 days post diode and emerald laser respectively but returned to normal by 21 days. Insulin-like growth factor Western ligand blotting demonstrated that a 34 kDa insulin-like growth factor binding protein was predominant in the pig vitreous; the levels of this binding protein followed an identical trend to those observed for insulin-like growth factor-I. No changes in vitreous levels of either basic fibroblast growth factor or epidermal growth factor were observed following laser treatment. Conclusions. Our results demonstrate a significant shift in the balance of intravitreal growth factors following retinal laser photocoagulation. Such changes may be pertinent to the regression of preretinal new vessels after laser photocoagulation.

AB - Purpose. To characterise changes in intravitreal growth factor profiles following retinal photocoagulation in the miniature pig. Methods. Miniature pig eyes underwent scatter photocoagulation by either diode infrared or emerald green laser. Animals were sacrificed at various times (up to 42 days) post-laser. The eyes were then removed and vitreous samples analysed for basic fibroblast growth factor, insulin-like growth factor-I and epidermal growth factor by radioimmunoassay, transforming growth factor-beta2 by ELISA and insulin-like growth factor binding proteins using Western ligand blotting. Results. Vitreous transforming growth factor-beta2 levels were decreased at 1 h post diode laser and at 4 and 7 days post emerald laser but returned to normal by 21 and 42 days respectively. Vitreous insulin-like growth factor-I levels increased at 4 and 7 days post diode and emerald laser respectively but returned to normal by 21 days. Insulin-like growth factor Western ligand blotting demonstrated that a 34 kDa insulin-like growth factor binding protein was predominant in the pig vitreous; the levels of this binding protein followed an identical trend to those observed for insulin-like growth factor-I. No changes in vitreous levels of either basic fibroblast growth factor or epidermal growth factor were observed following laser treatment. Conclusions. Our results demonstrate a significant shift in the balance of intravitreal growth factors following retinal laser photocoagulation. Such changes may be pertinent to the regression of preretinal new vessels after laser photocoagulation.

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