The Effects of Endogenous Sex Hormones and Acute Hypoxia on Vasoconstriction in Isolated Rat Pulmonary Artery Rings

Ketan M. Patel, Tim Lahm, Paul R. Crisostomo, Christine Herring, Troy Markel, Meijing Wang, Daniel R. Meldrum

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Studies have noted gender differences in various models but have not investigated whether hormone depletion will abolish these differences. Therefore, we measured isometric force displacement in normal males, castrated males, normal females, and ovarectomized females. Materials and methods: Adult male, adult female, castrated male, and ovarectomized female (250-350 g) Sprague Dawley rat pulmonary arteries (n = 7-8/group) were isolated and suspended in physiological organ baths. Force displacement was continuously recorded for 60 min of hypoxia. Data (mean ± SEM) was analyzed with two-way analysis of variance with post-hoc Bonferroni test or Student's t-test. Results: Maximum vasodilation of normal males was -79.47 ± 3.34%, while normal adult females exhibited a maximum vasodilation of -88.70 ± 6.21% (P = 0.8149). In addition, delayed, phase II vasoconstriction of male pulmonary arteries rings was 89.79 ± 7.25%, while adult females demonstrated a maximum phase II vasoconstriction of 95.90 ± 14.23% (P = 0.9342). Hormone depletion of males exhibited a maximum vasodilation of -70.45 ± 5.08% for castrated males as compared to -79.47 ± 3.34% for normal adult males (P = 0.3805). Castrated males exhibited a maximum phase II vasoconstriction of 86.20 ± 15.76% compared to 89.79 ± 7.25% exhibited by normal adult males (P = 0.9516). Conclusions: Hormone depletion in males and females did not alter pulmonary vasoreactivity in acute hypoxia.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
JournalJournal of Surgical Research
Volume146
Issue number1
DOIs
StatePublished - May 1 2008

Fingerprint

Gonadal Steroid Hormones
Vasoconstriction
Pulmonary Artery
Vasodilation
Hormones
Hypoxia
Baths
Sprague Dawley Rats
Analysis of Variance
Students

Keywords

  • estrogen
  • gender differences
  • hypoxic pulmonary vasoconstriction
  • sex hormones
  • testosterone

ASJC Scopus subject areas

  • Surgery

Cite this

The Effects of Endogenous Sex Hormones and Acute Hypoxia on Vasoconstriction in Isolated Rat Pulmonary Artery Rings. / Patel, Ketan M.; Lahm, Tim; Crisostomo, Paul R.; Herring, Christine; Markel, Troy; Wang, Meijing; Meldrum, Daniel R.

In: Journal of Surgical Research, Vol. 146, No. 1, 01.05.2008, p. 121-126.

Research output: Contribution to journalArticle

Patel, Ketan M. ; Lahm, Tim ; Crisostomo, Paul R. ; Herring, Christine ; Markel, Troy ; Wang, Meijing ; Meldrum, Daniel R. / The Effects of Endogenous Sex Hormones and Acute Hypoxia on Vasoconstriction in Isolated Rat Pulmonary Artery Rings. In: Journal of Surgical Research. 2008 ; Vol. 146, No. 1. pp. 121-126.
@article{f32a42a2578446a5a289b03db647536f,
title = "The Effects of Endogenous Sex Hormones and Acute Hypoxia on Vasoconstriction in Isolated Rat Pulmonary Artery Rings",
abstract = "Background: Studies have noted gender differences in various models but have not investigated whether hormone depletion will abolish these differences. Therefore, we measured isometric force displacement in normal males, castrated males, normal females, and ovarectomized females. Materials and methods: Adult male, adult female, castrated male, and ovarectomized female (250-350 g) Sprague Dawley rat pulmonary arteries (n = 7-8/group) were isolated and suspended in physiological organ baths. Force displacement was continuously recorded for 60 min of hypoxia. Data (mean ± SEM) was analyzed with two-way analysis of variance with post-hoc Bonferroni test or Student's t-test. Results: Maximum vasodilation of normal males was -79.47 ± 3.34{\%}, while normal adult females exhibited a maximum vasodilation of -88.70 ± 6.21{\%} (P = 0.8149). In addition, delayed, phase II vasoconstriction of male pulmonary arteries rings was 89.79 ± 7.25{\%}, while adult females demonstrated a maximum phase II vasoconstriction of 95.90 ± 14.23{\%} (P = 0.9342). Hormone depletion of males exhibited a maximum vasodilation of -70.45 ± 5.08{\%} for castrated males as compared to -79.47 ± 3.34{\%} for normal adult males (P = 0.3805). Castrated males exhibited a maximum phase II vasoconstriction of 86.20 ± 15.76{\%} compared to 89.79 ± 7.25{\%} exhibited by normal adult males (P = 0.9516). Conclusions: Hormone depletion in males and females did not alter pulmonary vasoreactivity in acute hypoxia.",
keywords = "estrogen, gender differences, hypoxic pulmonary vasoconstriction, sex hormones, testosterone",
author = "Patel, {Ketan M.} and Tim Lahm and Crisostomo, {Paul R.} and Christine Herring and Troy Markel and Meijing Wang and Meldrum, {Daniel R.}",
year = "2008",
month = "5",
day = "1",
doi = "10.1016/j.jss.2007.05.023",
language = "English",
volume = "146",
pages = "121--126",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - The Effects of Endogenous Sex Hormones and Acute Hypoxia on Vasoconstriction in Isolated Rat Pulmonary Artery Rings

AU - Patel, Ketan M.

AU - Lahm, Tim

AU - Crisostomo, Paul R.

AU - Herring, Christine

AU - Markel, Troy

AU - Wang, Meijing

AU - Meldrum, Daniel R.

PY - 2008/5/1

Y1 - 2008/5/1

N2 - Background: Studies have noted gender differences in various models but have not investigated whether hormone depletion will abolish these differences. Therefore, we measured isometric force displacement in normal males, castrated males, normal females, and ovarectomized females. Materials and methods: Adult male, adult female, castrated male, and ovarectomized female (250-350 g) Sprague Dawley rat pulmonary arteries (n = 7-8/group) were isolated and suspended in physiological organ baths. Force displacement was continuously recorded for 60 min of hypoxia. Data (mean ± SEM) was analyzed with two-way analysis of variance with post-hoc Bonferroni test or Student's t-test. Results: Maximum vasodilation of normal males was -79.47 ± 3.34%, while normal adult females exhibited a maximum vasodilation of -88.70 ± 6.21% (P = 0.8149). In addition, delayed, phase II vasoconstriction of male pulmonary arteries rings was 89.79 ± 7.25%, while adult females demonstrated a maximum phase II vasoconstriction of 95.90 ± 14.23% (P = 0.9342). Hormone depletion of males exhibited a maximum vasodilation of -70.45 ± 5.08% for castrated males as compared to -79.47 ± 3.34% for normal adult males (P = 0.3805). Castrated males exhibited a maximum phase II vasoconstriction of 86.20 ± 15.76% compared to 89.79 ± 7.25% exhibited by normal adult males (P = 0.9516). Conclusions: Hormone depletion in males and females did not alter pulmonary vasoreactivity in acute hypoxia.

AB - Background: Studies have noted gender differences in various models but have not investigated whether hormone depletion will abolish these differences. Therefore, we measured isometric force displacement in normal males, castrated males, normal females, and ovarectomized females. Materials and methods: Adult male, adult female, castrated male, and ovarectomized female (250-350 g) Sprague Dawley rat pulmonary arteries (n = 7-8/group) were isolated and suspended in physiological organ baths. Force displacement was continuously recorded for 60 min of hypoxia. Data (mean ± SEM) was analyzed with two-way analysis of variance with post-hoc Bonferroni test or Student's t-test. Results: Maximum vasodilation of normal males was -79.47 ± 3.34%, while normal adult females exhibited a maximum vasodilation of -88.70 ± 6.21% (P = 0.8149). In addition, delayed, phase II vasoconstriction of male pulmonary arteries rings was 89.79 ± 7.25%, while adult females demonstrated a maximum phase II vasoconstriction of 95.90 ± 14.23% (P = 0.9342). Hormone depletion of males exhibited a maximum vasodilation of -70.45 ± 5.08% for castrated males as compared to -79.47 ± 3.34% for normal adult males (P = 0.3805). Castrated males exhibited a maximum phase II vasoconstriction of 86.20 ± 15.76% compared to 89.79 ± 7.25% exhibited by normal adult males (P = 0.9516). Conclusions: Hormone depletion in males and females did not alter pulmonary vasoreactivity in acute hypoxia.

KW - estrogen

KW - gender differences

KW - hypoxic pulmonary vasoconstriction

KW - sex hormones

KW - testosterone

UR - http://www.scopus.com/inward/record.url?scp=41149117045&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41149117045&partnerID=8YFLogxK

U2 - 10.1016/j.jss.2007.05.023

DO - 10.1016/j.jss.2007.05.023

M3 - Article

VL - 146

SP - 121

EP - 126

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 1

ER -