The effects of glutathione depletion on the biodistribution of Cu(PTSM) in rats

Amy Barnhart-Bott; Mark A. Green

doi:10.1016/0883-2897(91)90095-3

The effects of glutathione depletion on the biodistribution of Cu(PTSM) in rats

Amy Barnhart-Bott, Mark A. Green

Research output: Contribution to journal › Article

10 Scopus citations

Abstract

The tissue retention of radiocopper afforded by intravenous injection of the proposed blood flow imaging agent, ⁶²Cu-labeled copper(II) pyruvaldehyde bis(N⁴-methylthiosemicarbazone) [Cu(PTSM)], is thought to result from reductive decomposition of the copper(II) complex by intracellular sulfhydryls (e.g. glutathione, GSH). To determine if the tissue uptake and retention of this tracer adequately measures perfusion in tissues containing altered GSH concentrations, the biodistribution of copper-67 labeled Cu(PTSM) was determined in GSH-depleted rats. Despite treatment to induce relatively large reductions in tissue GSH levels, it was found that only very small changes in the biodistribution of copper-labeled Cu(PTSM) occurred in the treated rats compared to untreated controls.

Original language	English (US)
Pages (from-to)	865-869
Number of pages	5
Journal	International Journal of Radiation Applications and Instrumentation.
Volume	18
Issue number	8
DOIs	https://doi.org/10.1016/0883-2897(91)90095-3
State	Published - 1991
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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Barnhart-Bott, A., & Green, M. A. (1991). The effects of glutathione depletion on the biodistribution of Cu(PTSM) in rats. International Journal of Radiation Applications and Instrumentation., 18(8), 865-869. https://doi.org/10.1016/0883-2897(91)90095-3

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abstract = "The tissue retention of radiocopper afforded by intravenous injection of the proposed blood flow imaging agent, 62Cu-labeled copper(II) pyruvaldehyde bis(N4-methylthiosemicarbazone) [Cu(PTSM)], is thought to result from reductive decomposition of the copper(II) complex by intracellular sulfhydryls (e.g. glutathione, GSH). To determine if the tissue uptake and retention of this tracer adequately measures perfusion in tissues containing altered GSH concentrations, the biodistribution of copper-67 labeled Cu(PTSM) was determined in GSH-depleted rats. Despite treatment to induce relatively large reductions in tissue GSH levels, it was found that only very small changes in the biodistribution of copper-labeled Cu(PTSM) occurred in the treated rats compared to untreated controls.",

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AB - The tissue retention of radiocopper afforded by intravenous injection of the proposed blood flow imaging agent, 62Cu-labeled copper(II) pyruvaldehyde bis(N4-methylthiosemicarbazone) [Cu(PTSM)], is thought to result from reductive decomposition of the copper(II) complex by intracellular sulfhydryls (e.g. glutathione, GSH). To determine if the tissue uptake and retention of this tracer adequately measures perfusion in tissues containing altered GSH concentrations, the biodistribution of copper-67 labeled Cu(PTSM) was determined in GSH-depleted rats. Despite treatment to induce relatively large reductions in tissue GSH levels, it was found that only very small changes in the biodistribution of copper-labeled Cu(PTSM) occurred in the treated rats compared to untreated controls.

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