Abstract
The tissue retention of radiocopper afforded by intravenous injection of the proposed blood flow imaging agent, 62Cu-labeled copper(II) pyruvaldehyde bis(N4-methylthiosemicarbazone) [Cu(PTSM)], is thought to result from reductive decomposition of the copper(II) complex by intracellular sulfhydryls (e.g. glutathione, GSH). To determine if the tissue uptake and retention of this tracer adequately measures perfusion in tissues containing altered GSH concentrations, the biodistribution of copper-67 labeled Cu(PTSM) was determined in GSH-depleted rats. Despite treatment to induce relatively large reductions in tissue GSH levels, it was found that only very small changes in the biodistribution of copper-labeled Cu(PTSM) occurred in the treated rats compared to untreated controls.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 865-869 |
| Number of pages | 5 |
| Journal | International Journal of Radiation Applications and Instrumentation. |
| Volume | 18 |
| Issue number | 8 |
| DOIs | |
| State | Published - 1991 |
| Externally published | Yes |
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The effects of glutathione depletion on the biodistribution of Cu(PTSM) in rats. / Barnhart-Bott, Amy; Green, Mark.
In: International Journal of Radiation Applications and Instrumentation., Vol. 18, No. 8, 1991, p. 865-869.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effects of glutathione depletion on the biodistribution of Cu(PTSM) in rats
AU - Barnhart-Bott, Amy
AU - Green, Mark
PY - 1991
Y1 - 1991
N2 - The tissue retention of radiocopper afforded by intravenous injection of the proposed blood flow imaging agent, 62Cu-labeled copper(II) pyruvaldehyde bis(N4-methylthiosemicarbazone) [Cu(PTSM)], is thought to result from reductive decomposition of the copper(II) complex by intracellular sulfhydryls (e.g. glutathione, GSH). To determine if the tissue uptake and retention of this tracer adequately measures perfusion in tissues containing altered GSH concentrations, the biodistribution of copper-67 labeled Cu(PTSM) was determined in GSH-depleted rats. Despite treatment to induce relatively large reductions in tissue GSH levels, it was found that only very small changes in the biodistribution of copper-labeled Cu(PTSM) occurred in the treated rats compared to untreated controls.
AB - The tissue retention of radiocopper afforded by intravenous injection of the proposed blood flow imaging agent, 62Cu-labeled copper(II) pyruvaldehyde bis(N4-methylthiosemicarbazone) [Cu(PTSM)], is thought to result from reductive decomposition of the copper(II) complex by intracellular sulfhydryls (e.g. glutathione, GSH). To determine if the tissue uptake and retention of this tracer adequately measures perfusion in tissues containing altered GSH concentrations, the biodistribution of copper-67 labeled Cu(PTSM) was determined in GSH-depleted rats. Despite treatment to induce relatively large reductions in tissue GSH levels, it was found that only very small changes in the biodistribution of copper-labeled Cu(PTSM) occurred in the treated rats compared to untreated controls.
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UR - http://www.scopus.com/inward/citedby.url?scp=44949285173&partnerID=8YFLogxK
U2 - 10.1016/0883-2897(91)90095-3
DO - 10.1016/0883-2897(91)90095-3
M3 - Article
VL - 18
SP - 865
EP - 869
JO - Nuclear Medicine and Biology
JF - Nuclear Medicine and Biology
SN - 0969-8051
IS - 8
ER -