The effects of radicular dentine treated with double antibiotic paste and ethylenediaminetetraacetic acid on the attachment and proliferation of dental pulp stem cells

Ki Wan Kim, Ghaeth H. Yassen, Ygal Ehrlich, Kenneth Spolnik, Jeffrey Platt, L. Windsor

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This study explored the effects of dentine treated with two concentrations of double antibiotic paste (DAP) and ethylenediaminetetraacetic acid (EDTA) on the attachment and proliferation of dental pulp stem cells (DPSCs). Materials and Methods: Radicular dentine samples were prepared with identical dimensions and randomized into six groups (n = 4). Four groups were treated with double antibiotic paste (DAP) at concentrations of 500 mg ml-1 or 1 mg ml-1 with or without EDTA. The other two groups were treated with EDTA only or received no treatment. DPSCs were seeded on each dentine sample (10 000 cells per sample). Lactate dehydrogenase activity assays were used to calculate the attached DPSCs after 1 day of incubation. Water soluble tetrazolium assays were performed to investigate DPSCs proliferation on the treated dentine samples after three additional days of incubation. Two-way anova followed by Tukey-Kramer tests was used for statistical analyses (α = 0.05). Results: Dentine treated with 1 or 500 mg ml-1 of DAP followed by EDTA caused significant increases in DPSCs attachment compared to the dentine treated with the DAP alone. The 500 mg ml-1 of DAP with or without EDTA caused significant reductions in DPSCs proliferation. However, the treatment of dentine with 1 mg ml-1 of DAP did not have significant negative effects on DPSCs proliferation regardless of the use of EDTA. Conclusion: The use of 1 mg ml-1 of DAP followed by 10 min of irrigation with EDTA in endodontic regeneration procedure may have no negative effects on the attachment and proliferation of DPSCs.

Original languageEnglish (US)
Pages (from-to)374-379
Number of pages6
JournalDental Traumatology
Volume31
Issue number5
DOIs
StatePublished - Oct 1 2015

Fingerprint

Dental Pulp
Dentin
Ointments
Edetic Acid
Stem Cells
Anti-Bacterial Agents
Cell Proliferation
Endodontics
L-Lactate Dehydrogenase
Regeneration
Water

Keywords

  • Dental pulp stem cells
  • Double antibiotic paste
  • Endodontic regeneration
  • Ethylenediaminetetraacetic acid
  • Radicular dentine

ASJC Scopus subject areas

  • Oral Surgery

Cite this

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title = "The effects of radicular dentine treated with double antibiotic paste and ethylenediaminetetraacetic acid on the attachment and proliferation of dental pulp stem cells",
abstract = "This study explored the effects of dentine treated with two concentrations of double antibiotic paste (DAP) and ethylenediaminetetraacetic acid (EDTA) on the attachment and proliferation of dental pulp stem cells (DPSCs). Materials and Methods: Radicular dentine samples were prepared with identical dimensions and randomized into six groups (n = 4). Four groups were treated with double antibiotic paste (DAP) at concentrations of 500 mg ml-1 or 1 mg ml-1 with or without EDTA. The other two groups were treated with EDTA only or received no treatment. DPSCs were seeded on each dentine sample (10 000 cells per sample). Lactate dehydrogenase activity assays were used to calculate the attached DPSCs after 1 day of incubation. Water soluble tetrazolium assays were performed to investigate DPSCs proliferation on the treated dentine samples after three additional days of incubation. Two-way anova followed by Tukey-Kramer tests was used for statistical analyses (α = 0.05). Results: Dentine treated with 1 or 500 mg ml-1 of DAP followed by EDTA caused significant increases in DPSCs attachment compared to the dentine treated with the DAP alone. The 500 mg ml-1 of DAP with or without EDTA caused significant reductions in DPSCs proliferation. However, the treatment of dentine with 1 mg ml-1 of DAP did not have significant negative effects on DPSCs proliferation regardless of the use of EDTA. Conclusion: The use of 1 mg ml-1 of DAP followed by 10 min of irrigation with EDTA in endodontic regeneration procedure may have no negative effects on the attachment and proliferation of DPSCs.",
keywords = "Dental pulp stem cells, Double antibiotic paste, Endodontic regeneration, Ethylenediaminetetraacetic acid, Radicular dentine",
author = "Kim, {Ki Wan} and Yassen, {Ghaeth H.} and Ygal Ehrlich and Kenneth Spolnik and Jeffrey Platt and L. Windsor",
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T1 - The effects of radicular dentine treated with double antibiotic paste and ethylenediaminetetraacetic acid on the attachment and proliferation of dental pulp stem cells

AU - Kim, Ki Wan

AU - Yassen, Ghaeth H.

AU - Ehrlich, Ygal

AU - Spolnik, Kenneth

AU - Platt, Jeffrey

AU - Windsor, L.

PY - 2015/10/1

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N2 - This study explored the effects of dentine treated with two concentrations of double antibiotic paste (DAP) and ethylenediaminetetraacetic acid (EDTA) on the attachment and proliferation of dental pulp stem cells (DPSCs). Materials and Methods: Radicular dentine samples were prepared with identical dimensions and randomized into six groups (n = 4). Four groups were treated with double antibiotic paste (DAP) at concentrations of 500 mg ml-1 or 1 mg ml-1 with or without EDTA. The other two groups were treated with EDTA only or received no treatment. DPSCs were seeded on each dentine sample (10 000 cells per sample). Lactate dehydrogenase activity assays were used to calculate the attached DPSCs after 1 day of incubation. Water soluble tetrazolium assays were performed to investigate DPSCs proliferation on the treated dentine samples after three additional days of incubation. Two-way anova followed by Tukey-Kramer tests was used for statistical analyses (α = 0.05). Results: Dentine treated with 1 or 500 mg ml-1 of DAP followed by EDTA caused significant increases in DPSCs attachment compared to the dentine treated with the DAP alone. The 500 mg ml-1 of DAP with or without EDTA caused significant reductions in DPSCs proliferation. However, the treatment of dentine with 1 mg ml-1 of DAP did not have significant negative effects on DPSCs proliferation regardless of the use of EDTA. Conclusion: The use of 1 mg ml-1 of DAP followed by 10 min of irrigation with EDTA in endodontic regeneration procedure may have no negative effects on the attachment and proliferation of DPSCs.

AB - This study explored the effects of dentine treated with two concentrations of double antibiotic paste (DAP) and ethylenediaminetetraacetic acid (EDTA) on the attachment and proliferation of dental pulp stem cells (DPSCs). Materials and Methods: Radicular dentine samples were prepared with identical dimensions and randomized into six groups (n = 4). Four groups were treated with double antibiotic paste (DAP) at concentrations of 500 mg ml-1 or 1 mg ml-1 with or without EDTA. The other two groups were treated with EDTA only or received no treatment. DPSCs were seeded on each dentine sample (10 000 cells per sample). Lactate dehydrogenase activity assays were used to calculate the attached DPSCs after 1 day of incubation. Water soluble tetrazolium assays were performed to investigate DPSCs proliferation on the treated dentine samples after three additional days of incubation. Two-way anova followed by Tukey-Kramer tests was used for statistical analyses (α = 0.05). Results: Dentine treated with 1 or 500 mg ml-1 of DAP followed by EDTA caused significant increases in DPSCs attachment compared to the dentine treated with the DAP alone. The 500 mg ml-1 of DAP with or without EDTA caused significant reductions in DPSCs proliferation. However, the treatment of dentine with 1 mg ml-1 of DAP did not have significant negative effects on DPSCs proliferation regardless of the use of EDTA. Conclusion: The use of 1 mg ml-1 of DAP followed by 10 min of irrigation with EDTA in endodontic regeneration procedure may have no negative effects on the attachment and proliferation of DPSCs.

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KW - Double antibiotic paste

KW - Endodontic regeneration

KW - Ethylenediaminetetraacetic acid

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