The effects of refractive index heterogeneity within kidney tissue on multiphoton fluorescence excitation microscopy

P. A. Young, S. G. Clendenon, J. M. Byars, Kenneth Dunn

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Although multiphoton fluorescence excitation microscopy has improved the depth at which useful fluorescence images can be collected in biological tissues, the reach of multiphoton fluorescence excitation microscopy is nonetheless limited by tissue scattering and spherical aberration. Scattering can be reduced in fixed samples by mounting in a medium whose refractive index closely matches that of the fixed material. Using optical 'clearing', the effects of refractive index heterogeneity on signal attenuation with depth are investigated. Quantitative measurements show that by mounting kidney tissue in a high refractive index medium, less than 50% of signal attenuates in 100 μm of depth.

Original languageEnglish
Pages (from-to)148-156
Number of pages9
JournalJournal of Microscopy
Volume242
Issue number2
DOIs
StatePublished - May 2011

Fingerprint

Multiphoton Fluorescence Microscopy
Refractometry
Kidney
Fluorescence

Keywords

  • Multiphoton fluorescence excitation microscopy
  • Refractive index heterogeneity
  • Refractive index mismatch
  • Scattering
  • Spherical aberration
  • Two-photon microscopy

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

The effects of refractive index heterogeneity within kidney tissue on multiphoton fluorescence excitation microscopy. / Young, P. A.; Clendenon, S. G.; Byars, J. M.; Dunn, Kenneth.

In: Journal of Microscopy, Vol. 242, No. 2, 05.2011, p. 148-156.

Research output: Contribution to journalArticle

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