The Effects of Tumor Necrosis Factor-α, Interleukin-1β, Interleukin-6, and Transforming Growth Factor-β1 on Pulp Fibroblast Mediated Collagen Degradation

Kessiri Wisithphrom, L. Jack Windsor

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Dental pulp destruction is believed to be regulated, in part, by the matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs). Cytokines are believed to be important in the pathogenesis of pulpitis. This study examined the effects that TNF-α, IL-1β, IL-6, and TGF-β1 have on the collagen degradation mediated by pulp fibroblasts utilizing a cell-mediated collagen degradation assay. Reverse transcriptase-polymerase chain reaction, Western blot analyses, and zymography were utilized to examine multiple MMPs and TIMPs. The collagen degradation mediated by these cells was stimulated by these cytokines. TNF-α, IL-1β, and IL-6 increased the mRNA and/or protein expression of MMP-1, MMP-2, and MMP-3. TGF-β1 decreased MMP-1 mRNA expression, while only slightly affecting the MMP-2 and MMP-3 mRNA and/or protein. These cytokines did not affect the expression of TIMP-1 or TIMP-2. These results suggest that these cytokines affect pulp destruction, in part, by differentially regulating the MMPs and TIMPs.

Original languageEnglish (US)
Pages (from-to)853-861
Number of pages9
JournalJournal of endodontics
Volume32
Issue number9
DOIs
StatePublished - Sep 1 2006

Keywords

  • Collagen degradation
  • cytokines
  • dental pulp fibroblasts
  • matrix metalloproteinases

ASJC Scopus subject areas

  • Dentistry(all)

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