The enterobacterial common antigen-like gene cluster of Haemophilus ducreyi contributes to virulence in humans

Keith E. Banks, Kate R. Fortney, Beth Baker, Steven D. Billings, Barry P. Katz, Robert S. Munson, Stanley M. Spinola

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Haemophilus ducreyi 35000HP contains a cluster of homologues of genes required for the synthesis of enterobacterial common antigen (ECA), suggesting that H. ducreyi may express a putative ECA-like glycoconjugate. WecA initiates the synthesis of ECA by transferring N-acetylglucosamine to undecaprenyl-P, to form lipid I. A wecA mutant (35000HPwecA) was constructed, and 5 volunteers were inoculated at 3 sites with fixed doses of 35000HP on one arm and at 3 sites with varying doses of 35000HPwecA on the other arm. 35000HPwecA caused pustules to form at 3 sites inoculated with a dose 2.5-fold higher than that of 35000HP. However, at sites inoculated with similar doses of 35000HP and 35000HPwecA, pustules developed at 46.7% (95% confidence interval [CI], 23.3%-70.0%) of 15 parent-strain sites and at 8.3% (95% CI, 0.01%-23.6%) of 12 mutant-strain sites (P = .013). Thus, the expression of wecA contributes to the ability of H. ducreyi to cause pustules in humans.

Original languageEnglish (US)
Pages (from-to)1531-1536
Number of pages6
JournalJournal of Infectious Diseases
Volume197
Issue number11
DOIs
StatePublished - Jun 1 2008

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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