The environmental stressor ultraviolet B radiation inhibits murine antitumor immunity through its ability to generate platelet-activating factor agonists

Ravi P. Sahu, Matthew J. Turner, Sonia C. Dasilva, Badri M. Rashid, Jesus A. Ocana, Susan Perkins, Raymond Konger, Christopher E. Touloukian, Mark Kaplan, Jeffrey Travers

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Ubiquitous pro-oxidative stressor ultraviolet B radiation (UVB) to human or mouse skin generates platelet-activating factor (PAF) and novel oxidatively modified glycerophosphocholines (Ox-GPCs) with PAF-receptor (PAF-R) agonistic activity. These lipids mediate systemic immunosuppression in a process involving IL-10. The current studies sought to determine the functional significance of UVB-mediated systemic immunosuppression in an established model of murine melanoma. We show that UVB irradiation augments B16F10 tumor growth and is dependent on host, but not melanoma cell; PAF-R-expression as UVB or the PAF-R agonist, carbamoyl PAF (CPAF), both promote B16F10 tumor growth in wild-type (WT) mice, independent of whether B16F10 cells express PAF-Rs, but do not augment tumor growth in Pafr -/- mice. UVB-mediated augmentation of experimental murine tumor growth was inhibited with antioxidants, demonstrating the importance of Ox-GPC PAF-R agonists produced non-enzymatically. Host immune cells are required as CPAF-induced augmentation of tumor growth which is not seen in immunodeficient NOD SCID mice. Finally, depleting antibodies against IL-10 in WT mice or depletion of CD25-positive cells in FoxP3 EGFP transgenic mice block UVB and/or CPAF-induced tumor growth supporting a requirement for IL-10 and Tregs in this process. These findings indicate that UVB-generated Ox-GPCs with PAF-R agonistic activity enhance experimental murine melanoma tumor growth through targeting host immune cells, most notably Tregs, to mediate systemic immunosuppression.

Original languageEnglish
Pages (from-to)1360-1367
Number of pages8
JournalCarcinogenesis
Volume33
Issue number7
DOIs
StatePublished - Jul 2012

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Platelet Activating Factor
Immunity
Radiation
Growth
Neoplasms
Interleukin-10
Immunosuppression
Melanoma
Inbred NOD Mouse
Experimental Melanomas
SCID Mice
Transgenic Mice
Antioxidants
Lipids
Skin
Antibodies

ASJC Scopus subject areas

  • Cancer Research

Cite this

The environmental stressor ultraviolet B radiation inhibits murine antitumor immunity through its ability to generate platelet-activating factor agonists. / Sahu, Ravi P.; Turner, Matthew J.; Dasilva, Sonia C.; Rashid, Badri M.; Ocana, Jesus A.; Perkins, Susan; Konger, Raymond; Touloukian, Christopher E.; Kaplan, Mark; Travers, Jeffrey.

In: Carcinogenesis, Vol. 33, No. 7, 07.2012, p. 1360-1367.

Research output: Contribution to journalArticle

Sahu, Ravi P. ; Turner, Matthew J. ; Dasilva, Sonia C. ; Rashid, Badri M. ; Ocana, Jesus A. ; Perkins, Susan ; Konger, Raymond ; Touloukian, Christopher E. ; Kaplan, Mark ; Travers, Jeffrey. / The environmental stressor ultraviolet B radiation inhibits murine antitumor immunity through its ability to generate platelet-activating factor agonists. In: Carcinogenesis. 2012 ; Vol. 33, No. 7. pp. 1360-1367.
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abstract = "Ubiquitous pro-oxidative stressor ultraviolet B radiation (UVB) to human or mouse skin generates platelet-activating factor (PAF) and novel oxidatively modified glycerophosphocholines (Ox-GPCs) with PAF-receptor (PAF-R) agonistic activity. These lipids mediate systemic immunosuppression in a process involving IL-10. The current studies sought to determine the functional significance of UVB-mediated systemic immunosuppression in an established model of murine melanoma. We show that UVB irradiation augments B16F10 tumor growth and is dependent on host, but not melanoma cell; PAF-R-expression as UVB or the PAF-R agonist, carbamoyl PAF (CPAF), both promote B16F10 tumor growth in wild-type (WT) mice, independent of whether B16F10 cells express PAF-Rs, but do not augment tumor growth in Pafr -/- mice. UVB-mediated augmentation of experimental murine tumor growth was inhibited with antioxidants, demonstrating the importance of Ox-GPC PAF-R agonists produced non-enzymatically. Host immune cells are required as CPAF-induced augmentation of tumor growth which is not seen in immunodeficient NOD SCID mice. Finally, depleting antibodies against IL-10 in WT mice or depletion of CD25-positive cells in FoxP3 EGFP transgenic mice block UVB and/or CPAF-induced tumor growth supporting a requirement for IL-10 and Tregs in this process. These findings indicate that UVB-generated Ox-GPCs with PAF-R agonistic activity enhance experimental murine melanoma tumor growth through targeting host immune cells, most notably Tregs, to mediate systemic immunosuppression.",
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