The expression of creatine kinase isoenzymes in neocortex of patients with neurodegenerative disorders

Alzheimer's and Pick's disease

M. Y. Aksenov, M. V. Aksenova, R. Payne, C. D. Smith, W. R. Markesbery, J. M. Carney

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Creatine kinase (CK) activity was found decreased in the brains of patients with Alzheimer's disease (AD) and Pick's disease (PD). However, the decrease of total CK activity in AD was more pronounced than in PD. Analysis of the activity of two CK isoforms, BCK and ubiquitous mitochondrial CK, demonstrated that the decrease of total CK activity in AD and PD was related to the decrease of BCK activity. The decline of CK activity both in AD and PD correlated well with the decline of the content of immunoreactive BCK in brain extracts. The BCK mRNA level in AD and PD was not significantly different from control patients and could not be the primary reason for decreases in CK content and activity. The decreased level of BCK in AD and PD brains might be caused by posttranscriptional events, which could affect the translation of BCK mRNA and/or lead to the inactivation and degradation of the enzyme. Because CK is sensitive to oxidative modification, it is possible that the changes observed in this study result from free radical damage.

Original languageEnglish (US)
Pages (from-to)458-465
Number of pages8
JournalExperimental Neurology
Volume146
Issue number2
DOIs
StatePublished - Aug 1997
Externally publishedYes

Fingerprint

Pick Disease of the Brain
Neocortex
Creatine Kinase
Neurodegenerative Diseases
Isoenzymes
Alzheimer Disease
Mitochondrial Form Creatine Kinase
Brain
Protein Biosynthesis
Free Radicals
Protein Isoforms
Messenger RNA
Enzymes

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

The expression of creatine kinase isoenzymes in neocortex of patients with neurodegenerative disorders : Alzheimer's and Pick's disease. / Aksenov, M. Y.; Aksenova, M. V.; Payne, R.; Smith, C. D.; Markesbery, W. R.; Carney, J. M.

In: Experimental Neurology, Vol. 146, No. 2, 08.1997, p. 458-465.

Research output: Contribution to journalArticle

Aksenov, M. Y. ; Aksenova, M. V. ; Payne, R. ; Smith, C. D. ; Markesbery, W. R. ; Carney, J. M. / The expression of creatine kinase isoenzymes in neocortex of patients with neurodegenerative disorders : Alzheimer's and Pick's disease. In: Experimental Neurology. 1997 ; Vol. 146, No. 2. pp. 458-465.
@article{100738ab87b040d992d558c54341b6af,
title = "The expression of creatine kinase isoenzymes in neocortex of patients with neurodegenerative disorders: Alzheimer's and Pick's disease",
abstract = "Creatine kinase (CK) activity was found decreased in the brains of patients with Alzheimer's disease (AD) and Pick's disease (PD). However, the decrease of total CK activity in AD was more pronounced than in PD. Analysis of the activity of two CK isoforms, BCK and ubiquitous mitochondrial CK, demonstrated that the decrease of total CK activity in AD and PD was related to the decrease of BCK activity. The decline of CK activity both in AD and PD correlated well with the decline of the content of immunoreactive BCK in brain extracts. The BCK mRNA level in AD and PD was not significantly different from control patients and could not be the primary reason for decreases in CK content and activity. The decreased level of BCK in AD and PD brains might be caused by posttranscriptional events, which could affect the translation of BCK mRNA and/or lead to the inactivation and degradation of the enzyme. Because CK is sensitive to oxidative modification, it is possible that the changes observed in this study result from free radical damage.",
author = "Aksenov, {M. Y.} and Aksenova, {M. V.} and R. Payne and Smith, {C. D.} and Markesbery, {W. R.} and Carney, {J. M.}",
year = "1997",
month = "8",
doi = "10.1006/exnr.1997.6550",
language = "English (US)",
volume = "146",
pages = "458--465",
journal = "Experimental Neurology",
issn = "0014-4886",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The expression of creatine kinase isoenzymes in neocortex of patients with neurodegenerative disorders

T2 - Alzheimer's and Pick's disease

AU - Aksenov, M. Y.

AU - Aksenova, M. V.

AU - Payne, R.

AU - Smith, C. D.

AU - Markesbery, W. R.

AU - Carney, J. M.

PY - 1997/8

Y1 - 1997/8

N2 - Creatine kinase (CK) activity was found decreased in the brains of patients with Alzheimer's disease (AD) and Pick's disease (PD). However, the decrease of total CK activity in AD was more pronounced than in PD. Analysis of the activity of two CK isoforms, BCK and ubiquitous mitochondrial CK, demonstrated that the decrease of total CK activity in AD and PD was related to the decrease of BCK activity. The decline of CK activity both in AD and PD correlated well with the decline of the content of immunoreactive BCK in brain extracts. The BCK mRNA level in AD and PD was not significantly different from control patients and could not be the primary reason for decreases in CK content and activity. The decreased level of BCK in AD and PD brains might be caused by posttranscriptional events, which could affect the translation of BCK mRNA and/or lead to the inactivation and degradation of the enzyme. Because CK is sensitive to oxidative modification, it is possible that the changes observed in this study result from free radical damage.

AB - Creatine kinase (CK) activity was found decreased in the brains of patients with Alzheimer's disease (AD) and Pick's disease (PD). However, the decrease of total CK activity in AD was more pronounced than in PD. Analysis of the activity of two CK isoforms, BCK and ubiquitous mitochondrial CK, demonstrated that the decrease of total CK activity in AD and PD was related to the decrease of BCK activity. The decline of CK activity both in AD and PD correlated well with the decline of the content of immunoreactive BCK in brain extracts. The BCK mRNA level in AD and PD was not significantly different from control patients and could not be the primary reason for decreases in CK content and activity. The decreased level of BCK in AD and PD brains might be caused by posttranscriptional events, which could affect the translation of BCK mRNA and/or lead to the inactivation and degradation of the enzyme. Because CK is sensitive to oxidative modification, it is possible that the changes observed in this study result from free radical damage.

UR - http://www.scopus.com/inward/record.url?scp=0031213840&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031213840&partnerID=8YFLogxK

U2 - 10.1006/exnr.1997.6550

DO - 10.1006/exnr.1997.6550

M3 - Article

VL - 146

SP - 458

EP - 465

JO - Experimental Neurology

JF - Experimental Neurology

SN - 0014-4886

IS - 2

ER -