The expression patterns of p53 and p16 and an analysis of a possible role of HPV in primary adenocarcinoma of the urinary bladder

Riley E. Alexander, Sean R. Williamson, Justin Richey, Antonio Lopez-Beltran, Rodolfo Montironi, Darrell Davidson, Muhammad Idrees, Carol L. Jones, Shaobo Zhang, Lisha Wang, Qiu Rao, Jose A. Pedrosa, Hristos Kaimakliotis, M. Francesca Monn, Michael Koch, Liang Cheng

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Primary adenocarcinoma of the urinary bladder is rare. The molecular and cellular events leading to its pathogenesis are not well delineated. The goal of this study was to investigate p53 and p16 expression, as well as HPV status, in a relatively large series of primary bladder adenocarcinomas. Materials and Methods: Thirty six cases of urinary bladder adenocarcinoma were chosen from participating institutions. The diagnosis and available clinical history were reviewed in each case. Immunostains for p53, p16 and HPV and high-risk and low-risk HPV-ISH were performed on all tumors. Results: Patients had an average age of 61 years with a male predominance (1.5:1 male:female ratio). The average tumor size in cystectomy specimens was 4.3 cm. Of the cases managed by transurethral resection, 40% were pT2 at the time of diagnosis. In cystectomy specimens, 77% were either pT3 or pT4. Strong nuclear p16 expression was seen in 67% of all cases and p53 expression was present in 58% of the cases. Expression of both markers was seen in 33% of cases. Expression of p16 or p53 alone was present in 12 (33%) and 9 (25%) cases, respectively. Neither marker was expressed in only 3 (8%) of the tumors. No significant correlation between clinical variables and any of the markers we studied was identified. No HPV infection was detected in any case. Conclusions: Expression of p53 and/or p16 is very common in urinary bladder adenocarcinoma. These findings implicate a high likelihood that alterations in these cell cycle proteins contribute to the pathogenesis of these tumors. Despite frequent immunohistochemical labeling for p16, no evidence of HPV infection was found.

Original languageEnglish
Article numbere95724
JournalPLoS One
Volume9
Issue number4
DOIs
StatePublished - Apr 21 2014

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adenocarcinoma
bladder
Tumors
Urinary Bladder
Adenocarcinoma
neoplasms
Cystectomy
Neoplasms
pathogenesis
Cell Cycle Proteins
resection
Infection
infection
Labeling
cell cycle
proteins
methodology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The expression patterns of p53 and p16 and an analysis of a possible role of HPV in primary adenocarcinoma of the urinary bladder. / Alexander, Riley E.; Williamson, Sean R.; Richey, Justin; Lopez-Beltran, Antonio; Montironi, Rodolfo; Davidson, Darrell; Idrees, Muhammad; Jones, Carol L.; Zhang, Shaobo; Wang, Lisha; Rao, Qiu; Pedrosa, Jose A.; Kaimakliotis, Hristos; Monn, M. Francesca; Koch, Michael; Cheng, Liang.

In: PLoS One, Vol. 9, No. 4, e95724, 21.04.2014.

Research output: Contribution to journalArticle

Alexander, Riley E. ; Williamson, Sean R. ; Richey, Justin ; Lopez-Beltran, Antonio ; Montironi, Rodolfo ; Davidson, Darrell ; Idrees, Muhammad ; Jones, Carol L. ; Zhang, Shaobo ; Wang, Lisha ; Rao, Qiu ; Pedrosa, Jose A. ; Kaimakliotis, Hristos ; Monn, M. Francesca ; Koch, Michael ; Cheng, Liang. / The expression patterns of p53 and p16 and an analysis of a possible role of HPV in primary adenocarcinoma of the urinary bladder. In: PLoS One. 2014 ; Vol. 9, No. 4.
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abstract = "Background: Primary adenocarcinoma of the urinary bladder is rare. The molecular and cellular events leading to its pathogenesis are not well delineated. The goal of this study was to investigate p53 and p16 expression, as well as HPV status, in a relatively large series of primary bladder adenocarcinomas. Materials and Methods: Thirty six cases of urinary bladder adenocarcinoma were chosen from participating institutions. The diagnosis and available clinical history were reviewed in each case. Immunostains for p53, p16 and HPV and high-risk and low-risk HPV-ISH were performed on all tumors. Results: Patients had an average age of 61 years with a male predominance (1.5:1 male:female ratio). The average tumor size in cystectomy specimens was 4.3 cm. Of the cases managed by transurethral resection, 40{\%} were pT2 at the time of diagnosis. In cystectomy specimens, 77{\%} were either pT3 or pT4. Strong nuclear p16 expression was seen in 67{\%} of all cases and p53 expression was present in 58{\%} of the cases. Expression of both markers was seen in 33{\%} of cases. Expression of p16 or p53 alone was present in 12 (33{\%}) and 9 (25{\%}) cases, respectively. Neither marker was expressed in only 3 (8{\%}) of the tumors. No significant correlation between clinical variables and any of the markers we studied was identified. No HPV infection was detected in any case. Conclusions: Expression of p53 and/or p16 is very common in urinary bladder adenocarcinoma. These findings implicate a high likelihood that alterations in these cell cycle proteins contribute to the pathogenesis of these tumors. Despite frequent immunohistochemical labeling for p16, no evidence of HPV infection was found.",
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T1 - The expression patterns of p53 and p16 and an analysis of a possible role of HPV in primary adenocarcinoma of the urinary bladder

AU - Alexander, Riley E.

AU - Williamson, Sean R.

AU - Richey, Justin

AU - Lopez-Beltran, Antonio

AU - Montironi, Rodolfo

AU - Davidson, Darrell

AU - Idrees, Muhammad

AU - Jones, Carol L.

AU - Zhang, Shaobo

AU - Wang, Lisha

AU - Rao, Qiu

AU - Pedrosa, Jose A.

AU - Kaimakliotis, Hristos

AU - Monn, M. Francesca

AU - Koch, Michael

AU - Cheng, Liang

PY - 2014/4/21

Y1 - 2014/4/21

N2 - Background: Primary adenocarcinoma of the urinary bladder is rare. The molecular and cellular events leading to its pathogenesis are not well delineated. The goal of this study was to investigate p53 and p16 expression, as well as HPV status, in a relatively large series of primary bladder adenocarcinomas. Materials and Methods: Thirty six cases of urinary bladder adenocarcinoma were chosen from participating institutions. The diagnosis and available clinical history were reviewed in each case. Immunostains for p53, p16 and HPV and high-risk and low-risk HPV-ISH were performed on all tumors. Results: Patients had an average age of 61 years with a male predominance (1.5:1 male:female ratio). The average tumor size in cystectomy specimens was 4.3 cm. Of the cases managed by transurethral resection, 40% were pT2 at the time of diagnosis. In cystectomy specimens, 77% were either pT3 or pT4. Strong nuclear p16 expression was seen in 67% of all cases and p53 expression was present in 58% of the cases. Expression of both markers was seen in 33% of cases. Expression of p16 or p53 alone was present in 12 (33%) and 9 (25%) cases, respectively. Neither marker was expressed in only 3 (8%) of the tumors. No significant correlation between clinical variables and any of the markers we studied was identified. No HPV infection was detected in any case. Conclusions: Expression of p53 and/or p16 is very common in urinary bladder adenocarcinoma. These findings implicate a high likelihood that alterations in these cell cycle proteins contribute to the pathogenesis of these tumors. Despite frequent immunohistochemical labeling for p16, no evidence of HPV infection was found.

AB - Background: Primary adenocarcinoma of the urinary bladder is rare. The molecular and cellular events leading to its pathogenesis are not well delineated. The goal of this study was to investigate p53 and p16 expression, as well as HPV status, in a relatively large series of primary bladder adenocarcinomas. Materials and Methods: Thirty six cases of urinary bladder adenocarcinoma were chosen from participating institutions. The diagnosis and available clinical history were reviewed in each case. Immunostains for p53, p16 and HPV and high-risk and low-risk HPV-ISH were performed on all tumors. Results: Patients had an average age of 61 years with a male predominance (1.5:1 male:female ratio). The average tumor size in cystectomy specimens was 4.3 cm. Of the cases managed by transurethral resection, 40% were pT2 at the time of diagnosis. In cystectomy specimens, 77% were either pT3 or pT4. Strong nuclear p16 expression was seen in 67% of all cases and p53 expression was present in 58% of the cases. Expression of both markers was seen in 33% of cases. Expression of p16 or p53 alone was present in 12 (33%) and 9 (25%) cases, respectively. Neither marker was expressed in only 3 (8%) of the tumors. No significant correlation between clinical variables and any of the markers we studied was identified. No HPV infection was detected in any case. Conclusions: Expression of p53 and/or p16 is very common in urinary bladder adenocarcinoma. These findings implicate a high likelihood that alterations in these cell cycle proteins contribute to the pathogenesis of these tumors. Despite frequent immunohistochemical labeling for p16, no evidence of HPV infection was found.

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