The eyelid margin

A transitional zone for 2 epithelial phenotypes

Shaohui Liu, Jing Li, Donald T.H. Tan, Roger W. Beuerman

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Objective: To investigate the differentiation profile of the epithelium of the eyelid margin in the primate. Methods: The expression of cytokeratins (CKs) CK1/ 10, CK4, CK14, CK5/8, and CK19; involucrin; connexin 43; filaggrin; and muscarinic receptor subtypes (m1-m5) on eyelid tissues from adult Macaca fascicularis (n = 3) was studied by immunohistochemistry. Results: Cytokeratin 1/10, CK4, CK19, filaggrin, and connexin 43 expression varied across the epithelium of the mucocutaneous junction (MCJ). At the MCJ, CK4-positive cells overlapped basal CK14-expressing cells. The meibomian gland duct and the MCJ revealed layers of CK14-positive cells. Cytokeratin 5/8 was expressed in the basal layer of the epidermis and conjunctiva, while involucrin-positive cells were superficial. The m2 was expressed throughout the conjunctiva; m3 was found in the basal cells of the skin, conjunctiva, and alveolar epithelial cells of the meibomian glands; and m4 was expressed in the suprabasal layers of the skin. Conclusions: The eyelid margin epithelium is formed from 2 different epithelial cell subpopulations with specific but overlapping distributions. The meibomian gland duct or MCJ may be a site of conjunctival progenitor cells. Clinical Relevance: This study provides a basis for understanding eyelid pathological conditions and eventually for developing methods for cellular reconstruction of the eyelid margin using expanded progenitor cells.

Original languageEnglish (US)
Pages (from-to)523-532
Number of pages10
JournalArchives of Ophthalmology
Volume125
Issue number4
DOIs
StatePublished - Apr 1 2007
Externally publishedYes

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Eyelids
Meibomian Glands
Conjunctiva
Phenotype
Connexin 43
Epithelium
Keratins
Stem Cells
Keratin-5
Muscarinic M1 Receptors
Keratin-8
Alveolar Epithelial Cells
Skin
Macaca fascicularis
Epidermis
Primates
Epithelial Cells
Immunohistochemistry
involucrin
filaggrin

ASJC Scopus subject areas

  • Ophthalmology

Cite this

The eyelid margin : A transitional zone for 2 epithelial phenotypes. / Liu, Shaohui; Li, Jing; Tan, Donald T.H.; Beuerman, Roger W.

In: Archives of Ophthalmology, Vol. 125, No. 4, 01.04.2007, p. 523-532.

Research output: Contribution to journalArticle

Liu, Shaohui ; Li, Jing ; Tan, Donald T.H. ; Beuerman, Roger W. / The eyelid margin : A transitional zone for 2 epithelial phenotypes. In: Archives of Ophthalmology. 2007 ; Vol. 125, No. 4. pp. 523-532.
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